Testicular Germ Cell Tumors

Methods and Protocols

Häftad, Engelska, 2021

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This volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology;

Basic Histopathologic Assessment of Germ Cell Tumors for Clinic and Research.- Clinical Applications of Immunohistochemistry in Germ Cell Tumors in Men.- Molecular Characterization of Testicular Germ Cell Tumors Using Tissue Microdissection.- Fluorescence In Situ Hybridization (FISH) Detection of Chromosomal 12p Anomalies in Testicular Germ Cell Tumors.- Germ Cell Tumor Cell Culture Techniques .- Three-Dimensional Cultivation of Germ Cell Cancer Cell Lines as Hanging Drops.- Cultivation of Testicular Germ Cell Cancer Cell Lines and Establishment of Gene-Edited Subclones using CRISPR/Cas9.- Evaluation of Chemotherapeutic Drugs for Treatment of (Cisplatin-Resistant) Germ Cell Cancer Cell Lines.- Assessing Homologous Recombination and Interstrand Crosslink Repair in Embryonal Carcinoma Testicular Germ Cell Tumor Cell Lines.- Production and Analysis of Human Primordial Germ Cell-Like Cells.- A Genetically Engineered Mouse Model of Malignant Testicular Germ Cell Tumors.- Targeted Methylation Analyses: From Bisulfite Treatment to Quantification.- Integrated Analysis of Germ Cell Tumors.- Use of Genome-Wide Association Studies to Evaluate Genetic Predisposition to Testicular Germ Cell Tumors.- A Circulating MicroRNA Panel for Malignant Germ Cell Tumor Diagnosis and Monitoring.- Detection of Circulating Tumor Cells (CTCs) in Patients with Testicular Germ Cell Tumors.- Developing and Using a Data Commons for Understanding the Molecular Characteristics of Germ Cell Tumors.