Techniques in Animal Cytogenetics

Inbunden, Engelska, 2000

AvHelene Hayes,P. Popescu,Paul Popescu,Helene Hayes,Bernard Dutrillaux

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The theoretical background of cytogenetics as well as the practical methods to investigate chromosomes from Drosophila to mammals and humans are presented in this book. Theory and practical protocols on the following topics are included: Cell culture methods, chromosome preparation and banding techniques, radioactive and fluorescent in situ hybridisation (FISH) methods and their derivates (chromosome painting), meiotic chromosome methods using light and electronic microscopy, the polytenic (Drosophila) and lampbrush (urodeles) chromosomes as well as flow and slit scan cytometry. The editors, all at INRA, played a prominent part in the development of cytogenetics. Paul Popescu, a specialist for domestic animals worked with gene mapping, Helene Hayes, together with Popescu showed the homology of bovine and human chromosome disorders, and Bernard Dutrilaux focused on human cytogenetics.

Produktinformation

Utgivningsdatum2000-08-15
Mått155 x 235 x 21 mm
Vikt606 g
FormatInbunden
SpråkEngelska
SeriePrinciples and Practice
Antal sidor230
Upplaga2000
FörlagSpringer-Verlag Berlin and Heidelberg GmbH & Co. KG
ISBN9783540667377
ÖversättarePopescu, R.

Tillhör följande kategorier

Fysiologi inom Medicin
Medicinsk genetik inom Medicin
Biokemi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik
Agronomi inom Naturvetenskap och teknik

I Preparation of Chromosome Spreads.- I.1 Cell culture techniques.- I.2 Preparation of chromosomes in prophase or prometaphase.- I.3 Direct techniques and very short term development cultures.- I.4 Preparation of chromosome spreads.- I.5 Staining techniques of chromosome spreads.- II Chromosome Banding Techniques.- II. 1 Introduction.- II.2 Techniques based on DNA structure.- II.3 Banding techniques based on DNA replication.- II.4 Techniques of chromosome differentiation based on DNA base composition.- II.5 Heterochromatin staining.- II.6 Staining of nucleolar organiser regions NOR.- II.7 Techniques of sequential banding.- III In Situ Hybridisation Techniques.- III.1 Introduction.- III.2 Methods.- III.3 Remarks on other applications of in situ hybridization.- IV Methods of Germ Cells Study.- IV.1 Meiosis in male.- IV.2 Meiosis in the mammalian female.- IV.3 Study of the spermatozoa by interspecific in vitro fertilisation (insemination).- V The Lampbrush Chromosomes of Amphibians.- V.1Introduction: The basis of lampbrush chromosomes mapping.- V.2 Technique for the preparation of lampbrush chromosomes for light microscopy.- V.3 Preparation of lampbrush chromosomes for electron microscopy.- V.4 Preparation of mitotic chromosomes.- V.5 Analysis of lampbrush chromosomes.- V.6 The importance of the lampbrush chromosomes.- VI Techniques for the study of Drosophila Chromosomes.- VI.1 Mitotic chromosomes.- VI.2 Polythene chromosomes.- VII Techniques for the stuey of Interphase Nucleus.- VII.1 Sex chromatin examination.- VII.2 Released chromatin (Chromatin halo).- VII.3 In situ hybridisation of interphasic nuclei.- VIII Application of Flow Cytometry and Slit-Scan Fluorometry in Analysis and Sorting of Mammalian Chromosomes.- VIII.1 Introduction.- VIII.2 Principles of the flow cytometry.- VIII.3 Methods of chromosome preparation and staining.- VIII.4 Measurements and evaluation in flow cytometry.- VIII.5 In situ hybridisation to chromosomes in suspension.- S Solutions for chromosome staining and banding techniques.- M Miscellaneous.- L Solutions for lampbrush chromosomes.- F Solutions for flow and slit scan cytometry.- H Solutions for in situ hybridization.- CM Culture media.- SS Stock solutions.- B Buffer solutions.- V Solutions for in vivo treatments.- References.