Systems Biology of Alzheimer's Disease

Stephen G. OliverDepartment of Biochemistry & Cambridge Systems Biology CentreUniversity of CambridgeCambridge UKsteve.oliver@bioc.cam.ac.uk Juan I. CastrilloDepartment of Biochemistry & Cambridge Systems Biology CentreUniversity of CambridgeCambridge UK Genetadi Biotech S.L. PraqueTecnologico de BizkaiaDerio, Bizkaia, Spainjicd28@yahoo.co.uk   jic28@genetadi.com

• Alzheimer’s as a Systems-Level Disease Involving the Interplay of Multiple Cellular Networks.- Application of Systems Theory in Longitudinal Studies on the Origin and Progression of Alzheimer’s Disease.- The APP Proteolytic System and Its Interactions with Dynamic Networks in Alzheimer’s Disease.- Effects of Mild and Severe Oxidative Stress on BACE1 Expression and APP Amyloidogenic Processing.- Advanced Assay Monitoring APP-Carboxyl-Terminal Fragments as Markers of APP Processing in Alzheimer Disease Mouse Models.- Advanced Optical Super-Resolution Method and Techniques for the Study of β-Amyloid Aggregation In Vitro and In Vivo.- Protocols for Monitoring the Development of Tau Pathology in Alzheimer’s Disease.- LC3-II Tagging and Western Blotting for Monitoring Autophagic Activity in Mammalian Cells.- Advanced Mitochondrial Respiration Assay for Evaluation of Mitochondrial Dysfunction in Alzheimer’s Disease.- Analysis of Microglial Proliferation in Alzheimer’s Disease.- Yeast as aModel for Alzheimer’s Disease: Latest Studies and Advanced Strategies.- Yeast as a Model for Studies on Aβ Aggregation Toxicity in Alzheimer’s Disease, Autophagic Responses, and Drug Screening.- Drosophila melanogaster as a Model for Studies on the Early Stages of Alzheimer’s Disease.- Chronic Mild Stress Assay Leading to Early Onset and Propagation of Alzheimer’s Disease Phenotype in Mouse Models.- Gene Expression Studies on Human Trisomy 21 iPSCs and Neurons: Towards Mechanisms Underlying Down’s Syndrome and Early Alzheimer’s Disease-Like Pathologies.- Cortical Differentiation of Human Pluripotent Cells for In Vitro Modeling of Alzheimer’s Disease.- Next Generation Sequencing in Alzheimer’s Disease.- Pooled-DNA Sequencing for Elucidating New Genomic Risk Factors, Rare Variants Underlying Alzheimer’s Disease.- New Genome-Wide Methods for Elucidation of Candidate Copy Number Variations (CNVs) Contributing to Alzheimer’s Disease Heritability.- RNA-Sequencing to Elucidate Early Patterns of Dysregulation Underlying the Onset of Alzheimer’s Disease.- Systems Biology Approaches to the Study of Biological Networks Underlying Alzheimer’s Disease: Role of miRNAs.- The Emerging Role of Metalloproteomics in Alzheimer’s Disease Research.- Redox Proteomics in Human Biofluids: Sample Preparation, Separation and Immunochemical Tagging for Analysis of Protein Oxidation.- Advanced Shotgun Lipidomics for Characterization of Altered Lipid Patterns in Neurodegenerative Diseases and Brain Injury.- AlzPathway, an Updated Map of Curated Signaling Pathways: Towards Deciphering Alzheimer’s Disease Pathogenesis.- A Computational Network Biology Approach to Uncover Novel Genes Related to Alzheimer’s Disease.- Network Approaches to the Understanding of Alzheimer’s Disease: From Model Organisms to Humans.- Characterization of Genetic Networks Associated with Alzheimer's Disease.- Network-Based Analysis for Uncovering Mechanisms Underlying Alzheimer’s Disease.- The SDREM Method for Reconstructing Signaling and Regulatory Response Networks: Applications for Studying Disease Progression.- Advanced Neuroimaging Methods Towards Characterization of Early Stages of Alzheimer’s Disease.- Plasma Proteomics Biomarkers in Alzheimer's Disease: Latest Advances and Challenges.- A Practical Guide For Exploring Opportunities of Repurposing Drugs for CNS Diseases in Systems Biology.

"The book represents a very useful introduction to Systems Biology as the approach necessary to obtain a global view of the dysregulation of pathways involved in AD. In the need for standardization of techniques, the methods described in this book provide a step forward in the definition of common guidelines leading to the reproducible and dependable results necessary for a better understanding of the pathophysiology of AD." (Dr. Alessandro Villa, Journal of Alzheimer's Disease, Vol. 50, 2016)