Del 0 - Methods in Molecular Biology

Synthetic mRNA

Production, Introduction Into Cells, and Physiological Consequences

Häftad, Engelska, 2018

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This volumepresents detailed laboratory protocols for invitro synthesis of mRNA with favorable properties, its introduction intocells by a variety of techniques, and the measurement of physiological andclinical consequences such as protein replacement and cancer immunotherapy.

Produktinformation

Utgivningsdatum2018-05-26
Mått178 x 254 x 19 mm
Vikt626 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor320
FörlagHumana Press Inc.
ISBN9781493980987

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Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequences.- Synthetic Capped mRNAs for Cap-Specific Photo-Crosslinking Experiments.- Enzymatic Modification of 5’-Capped RNA and Subsequent Labeling by Click Chemistry.- Preparation of Functional, Fluorescently Labeled mRNA Capped with Anthraniloyl-m7GpppG.- Intronless b-Globin Reporter: A Tool for Studying Nuclear RNA Stability Elements.- Synthetic mRNA with Superior Properties that Mimics the Intracellular Fates of Natural Histone mRNA.- Engineering WT1-Encoding mRNA to Increase Translational Efficiency in Dendritic Cells.- Electroporation of Alphavirus RNA Translational Reporters into Fibroblastic and Myeloid Cells as a Tool to Study the Innate Immune System.- GMP-Grade mRNA Electroporation of Dendritic Cells for Clinical Use.- Large Scale mRNA Transfection of Dendritic Cells by Electroporation in Continuous Flow Systems.- FLT3 Ligand as a Molecular Adjuvant for Naked RNA Vaccines.- Transfecting Human Monocytes with RNA.- In Vitro Synthesis, Delivery and Bioavailability of Exogenous mRNA in Gene Transfer Mediated byPiggyBac Transposition.- Transfection of Human Keratinocytes with Nucleoside-Modified mRNA Encoding CPD-Photolyase to Repair DNA Damage.- Delivery of Synthetic mRNA Encoding FOXP3 Antigen into Dendritic Cells for Inflammatory Breast Cancer Immunotherapy.- Immune Monitoring using mRNA-Transfected Dendritic Cells.- Transfection of Tumor-Infiltrating T Cells with mRNA Encoding CXCR2.- mRNA Electroporation of Dendritic Cells with WT1, Survivin and TriMix (A Mixture of caTLR4, CD40L, and CD70).- Redirecting T Cell Specificity Using T Cell Receptor Messenger RNA Electroporation.- Measuring Hematocrit in Mice Injected with In Vitro-Transcribed Erythropoietin mRNA.- Genetic Modification of Human Pancreatic Progenitor Cells through Modified mRNA.