Del 497 - Methods in Enzymology

Synthetic Biology, Part A

Methods for Part/Device Characterization and Chassis Engineering

Inbunden, Engelska, 2011

Av Chris Voigt

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Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation.

Encompasses a variety of different approaches, methodologies and disciplines
Split into 2 parts and covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation

Produktinformation

Utgivningsdatum2011-07-08
Mått152 x 229 x undefined mm
Vikt1 270 g
FormatInbunden
SpråkEngelska
SerieMethods in Enzymology
Antal sidor714
FörlagElsevier Science
ISBN9780123850751

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Biokemisk teknik inom Naturvetenskap och teknik

Section I. Measuring and Engineering Central Dogma Processes Sequence-specificity and Energy Landscapes of DNA-binding MoleculesJoshua R. Tietjen, Leslie J. Donato, Devesh Bhimisaria and Aseem Z. AnsariPromoter Reliability in Modular Transcriptional NetworksRajat Anand, Navneet Rai and Mukund ThattaiThe Analysis of ChIP-seq DataWenxiu Ma and Wing Hung WongUsing DNA Microarrays to Assay Part FunctionVirgil A. Rhodius and Carol A. GrossOrthogonal gene expression in Escherichia coliWenlin An and Jason ChinDirected evolution of promoters and tandem gene arrays for customizing RNA synthesis rates and regulationKeith E.J. Tyo, Elke Nevoigt and Gregory StephanopoulosSection II. Device and System Design, Optimization, and DebuggingDesign and Connection of Robust Genetic Circuits Ron WeissEngineering RNAi circuitsYaakov BenensonFrom SELEX to cell: Dual selections for synthetic riboswitchesJoy Sinha, Shana Topp, and Justin P. GallivanUsing noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in mammalian cellsJeffrey V. Wong, Guang Yao, Joseph R. Nevins and Lingchong YouDe novo design and construction of an inducible gene expression system in mammalian cellsMaria Karlsson, Wilfried Weber and Martin FusseneggerBioBuilding: Using banana-scented bacteria to teach synthetic biologyJames Dixon and Natalie KuldellSection III. Device Measurement, Optimization, and DebuggingUse of Fluorescence Microscopy to Analyze Genetic Circuit DynamicsGürol SüelMicrofluidics for synthetic biology: From design to executionM. S. Ferry, I. A. Razinkov, J. HastyPlate-based assays for light-regulated gene expression systemsJeffrey J. TaborSpatiotemporal control of small GTPases with light using the LOV domainYi I. Wu, Xiaobo Wang, Li He, Denise Montell and Klaus M. HahnLight Control of Plasma Membrane Recruitment using the Phy—PIF systemJared E. Toettcher, Delquin Gong, Wendell A. Lim, Orion D. WeinerSynthetic Physiology: Strategies for Adapting Tools from Nature for Genetically-Targeted Control of Fast Biological ProcessesEd BoydenSection IV. Devices for Metabolic EngineeringMetabolic Pathway Flux Enhancement by Synthetic Protein ScaffoldingJohn E. Dueber and Weston R. WhitakerA Synthetic Iterative Pathway for ketoacid elongationC. R. Shen, J. C. LiaoSection V. Expanding ChassesSynthetic Biology in Streptomyces bacteriaMarnix Medema, Rainer Breitling and Eriko TakanoMethods for Engineering Sulfate Reducing Bacteria of the Genus DesulfovibrioKimberly L. Keller, Judy D. Wall and Swapnil ChhabraModification of the Genome of Rhodobacter sphaeroides and Construction of Synthetic OperonsPaul R. Jaschke, Rafael G. Saer, Stephan Noll and J. Thomas BeattySynthetic Biology in Cyanobacteria: Engineering and Analyzing Novel FunctionsThorsten Heidorn, Daniel Camsund, Hsin-Ho Huang, Pia Lindberg, Paulo Oliveira, Karin Stensjö, and Peter LindbladDeveloping a Synthetic Signal Transduction System in PlantsKevin J. Morey, Mauricio S. Antunes, Kirk D. Albrecht, Tessa A. Bowen, Jared F. Troupe, Keira L. Havens and June I. MedfordLentiviral Vectors to Study Stochastic Noise in Gene Expression Kate Franz, Abhyudai Singh, and Leor S. Weinberger