Del 1396 - Methods in Molecular Biology

Superantigens

Methods and Protocols

Häftad, Engelska, 2019

Av Amanda J. Brosnahan

1 389 kr

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This volume combines protocols that encompass the true variety of investigation done on superantigens in the fields of microbiology, immunology, molecular biology, biochemistry, and cellular biology, with a strong focus on disease models utilized to determine the role of superantigens in human disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and cutting-edge, Superantigens: Methods and Protocols contains a detailed and wide breadth of subject coverage that any scientist, clinician, or industry professional interested in this field will find valuable.

Produktinformation

Utgivningsdatum2019-03-29
Mått178 x 254 x 14 mm
Vikt460 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor228
FörlagHumana Press Inc.
ISBN9781493980260

Tillhör följande kategorier

Onkologi inom Medicin

Animal Models Used to Study Superantigen-Mediated Diseases.- Identification, Purification, and Characterization of Staphylococcal Superantigens.- Strain Discrimination of Staphylococcus Aureus Using Superantigen Profiles.- Crystallization and Structure Determination of Superantigens and Immune Receptor Complexes.- Lipopolysaccharide-Induced Toxic Shock Syndrome in Rabbits.- Aortic Valve Damage for the Study of Left-Sided, Native Valve Infective Endocarditis in Rabbits.- Rabbit Model for Superantigen-Mediated Lethal Pulmonary Disease.- Nasopharyngeal Infection of Mice with Streptococcus Pyogenes and In Vivo Detection of Superantigen Activity.- Mini-Osmotic Pump Infusion Model to Investigate the Systemic Effects of Chronic Continuous Exposure to Staphylococcal Superantigen in Mice.- A Sub-Lethal Swine Model for Defining In Vivo Superantigen-Induced Responses Following Exposure to Staphylococcal Enterotoxin B.- Monkey Feeding Assay for Testing Emetic Activity of Staphylococcal Enterotoxin.- Use of an Ex-Vivo Porcine Mucosal Model to Study Superantigen Penetration.- Protocol for Examining Human Vaginal Epithelial Cell Signaling in Response to Staphylococcal Superantigens.- Organ Culture as a Model System for Studies on Enterotoxin Interactions with the Intestinal Epithelium.- Quantification of a Selective Expansion of T Cell Receptor Vβ by Superantigen Using Real Time PCR.- Induction of Human Regulatory T Cells with Bacterial Superantigens.- Construction of Recombinant Single Chain Variable Fragment (ScFv) Antibody Against Superantigen for Immunodetection Using Antibody Phage Display Technology.