Super-Resolution Imaging in Biomedicine

Häftad, Engelska, 2021

Av Alberto Diaspro, Marc A. M. J. van Zandvoort, Marc A. M. J. Van Zandvoort, Marc A. M. J. van Zandvoort

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This book encompasses the full breadth of the super-resolution imaging field, representing modern techniques that exceed the traditional diffraction limit, thereby opening up new applications in biomedicine. It shows readers how to use the new tools to increase resolution in sub-nanometer-scale images of living cells and tissue, which leads to new information about molecules, pathways and dynamics. The book highlights the advantages and disadvantages of the techniques, and gives state-of-the-art examples of applications using microscopes currently available on the market. It covers key techniques such as stimulated emission depletion (STED), structured illumination microscopy (SSIM), photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM). It will be a useful reference for biomedical researchers who want to work with super-resolution imaging, learn the proper technique for their application, and simultaneously obtain a solid footing in other techniques.

Produktinformation

Utgivningsdatum2021-03-31
Mått178 x 254 x 24 mm
Vikt1 211 g
FormatHäftad
SpråkEngelska
SerieSeries in Cellular and Clinical Imaging
Antal sidor448
FörlagTaylor & Francis Ltd
ISBN9780367782740

Tillhör följande kategorier

Tillämpad fysik inom Naturvetenskap och teknik
Fysik inom Naturvetenskap och teknik
Biomedicinsk teknik inom Medicin

Alberto Diaspro, Ph.D, is a professor of applied physics and head of the Nanophysics Unit of the Italian Institute of Technology. He also founded the Laboratory for Advanced Microscopy, Bioimaging, and Spectroscopy (LAMBS).Marc A.M.J. van Zandvoort, Ph.D, is the leader of the Two-Photon Core Facility in Aachen and head of the Advanced Microscopy Unit in the Department of Genetics and Cell Biology at Maastricht University, Netherlands.

Overview of Super-Resolution Technologies. Introduction to the Main Techniques - (F)PALM/(d)STORM, STED, SIM. Super-Resolution Imaging of Single Molecules. Image Processing for Super-Resolution. Super-Resolution Fluorescent Probes. The Methods. Principles and Techniques for STED; SIM/SSIM; SOFI. Single-Molecule Localization Microscopy - PALM, (F)PALM, (d)STORM): Principles, Probes, and Algorithms. Principles and Techniques for SPDM; GSDIM. Applications in Biology and Medicine. Synaptic Protein Distributions Studied with (d)STORM/(F)PALM. Studies of Chromosomal Organization at Super Resolution. Nanoscale Organization of RNA Polymerase Using PALM. Super-Resolution Microscopy of Protein Machines in Bacteria. Super-Resolution Microscopy of Living Biological Nanostructures. SIM for the Study of Mitochondrial Dynamics in 3D. Neurobiological Applications Using STED. STED in HIV Research. Super-Resolution Imaging in Cardiovascular Research. Quantitative Super-Resolution Imaging of Proteins.

"an outstanding book suitable for people with interests in the biomedical applications of super-resolution microscopy…. The chapter on structured-light microscopy is another gem, with its exposition of theory, instrumentation and limitations. The warning of artifacts in the specimen preparation, the image acquisition, as well as image analysis and interpretation, permeate the book’s content…. The figures are all high quality" –Optics & Photonics News (Mar 2017), review by Barry R. Masters