Structural Proteomics

High-Throughput Methods

Inbunden, Engelska, 2021

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This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins.

Produktinformation

Utgivningsdatum2021-05-06
Mått178 x 254 x 25 mm
Vikt867 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor344
Upplaga3
FörlagSpringer-Verlag New York Inc.
ISBN9781071614051

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Kemi inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik

The Protein Data Bank Archive.- Computational Methods for the Elucidation of Protein Structure and Interactions.- Methods for Molecular Modeling of Protein Complexes.- High-Level Production of Recombinant Eukaryotic Proteins from Mammalian Cells Using Lentivirus.- Transient Transfection and Expression of Eukaryotic Membrane Proteins in Expi293F Cells and Their Screening on a Small-Scale: Application for Structural Studies.- Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells.- SynBac: Enhanced Baculovirus Genomes by Iterative Recombineering.- Gene Tagging with the CRISPR-Cas9 System to Facilitate Macromolecular Complex Purification.- Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems.- Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins.- Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources.- From Tube to Structure:SPA Cryo-EM Workflow Using Apoferritin as an Example.- Image Processing in Cryo-Electron Microscopy of Single Particles: The Power of Combining Methods.- Setup and Troubleshooting of Volta Phase Plate Cryo-EM Data Collection.- Cryo-Focused Ion Beam Lamella Preparation Protocol for In Situ Structural Biology.- Protein and Small Molecule Structure Determination by the Cryo-EM Method MicroED.