• Optimal Conditions for Streptococcus pneumoniae Culture: In Solid and Liquid Media.- Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell.- Immunofluorescent Staining and High-Resolution Microscopy to Study the Pneumococcal Cell.- Construction of Fluorescent Pneumococci for In Vivo Imaging and Labeling of the Chromosome.- High-Resolution and Super-Resolution Immunofluorescent Microscopy Ex Vivo to Study Pneumococcal Interactions with the Host.- Natural Genetic Transformation: A Direct Route to Easy Insertion of Chimeric Genes into the Pneumococcal Chromosome.- Gene Expression Analysis in the Pneumococcus.- Transcriptional Knockdown in Pneumococci using CRISPR Interference.- Protein Expression Analysis by Western Blot and Protein-Protein Interactions.- Mass Spectrometry to Study the Bacterial Proteome from a Single Colony.- Bead-Based Flow Cytometric Cell Counting of Live and Dead Bacteria.- In Vitro Adhesion, Invasion, and Transcytosis of Streptococcus pneumonia with Host Cells.- Growing and Characterizing Biofilms Formed by Streptococcus pneumoniae.- In Vivo Mouse Models to Study How Pneumococci Interact with the Host and Cause Invasive Pneumococcal Disease.- Two-Photon Intravital Imaging of Leukocytes in the Trachea during Pneumococcal Infection.- IVIS Spectrum CT to Image the Progression of Pneumococcal Infections In Vivo.- The Pneumococcus and Its Critical Role in Public Health.- The Epidemiology and Biostatistics of Pneumococcus.

“The audience includes students, researchers, and microbiologists, as well as others in the fields of public health, epidemiology, and biostatistics related to Streptococcus pneumoniae. ... This is an excellent book on Streptococcus pneumoniae, with additional coverage of the related public health, epidemiology, and biostatistics aspects. I am sure that the information in this book will be of much help and interest to the intended audience.” (Omer Iqbal, Doody's Book Reviews, December 7, 2019)