This book constitutes the thoroughly refereed post-conference proceedings of the 16th International Conference on Smart Card Research and Advanced Applications, CARDIS 2017, held in Lugano, Switzerland, in November 2017.The 14 revised full papers presented together with 2 abstracts of invited talks in this book were carefully reviewed and selected from 48 submissions. CARDIS has provided a space for security experts from industry and academia to exchange on security of smart cards and related applications.