Signal Transduction Immunohistochemistry

• Overview of the Generation, Validation, and Application of Phosphosite-Specific Antibodies.- Selection and Validation of Antibodies for Signal Transduction Immunohistochemistry.- An Overview of Western Blotting for Determining Antibody Specificities for Immunohistochemistry.- Optimized Protocol to Make Phospho-Specific Antibodies that Work.- Methodology and Technology for Stabilization of Specific States of Signal Transduction Proteins.- An Enhanced Antigen Retrieval Protocol for Immunohistochemical Staining of Formalin-Fixed, Paraffin-Embedded Tissues.- Imaging Techniques in Signal Transduction IHC.- Practical Considerations of Image Analysis and Quantification of Signal Transduction IHC Staining.- Flow Cytometric Analysis of Cell Signaling Proteins.- CytoSys: A Tool for Extracting Cell Cycle-Related Expression Dynamics from Static Data.- Signaling Events Initiated by Kappa Opioid Receptor Activation: Quantification and Immunolocalization Using Phospho-Selective KOR, p38-MAPK, and KIR 3.1 Antibodies.- Immunohistochemical Assessment of Signal Transduction and Cell-Cycle Networks in Neural Tumors.- Novel Multi-Color Immunofluorescence Technique Using Primary Antibodies Raised in the Same Host Species.- Activation and Differentiation of Mesenchymal Stem Cells.- Double In situ Detection of Sonic Hedgehog mRNA and pMAPK Protein in Examining the Cell Proliferation Signaling Pathway in Mouse Embryo.- Identifying Intracellular Sites of Eicosanoid Lipid Mediator Synthesis with EicosaCell Assays.- Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads.

From the reviews:“Each of us daily using immunohistochemical protocols to reveal targets either useful for research or diagnostic aims will surely wonder by which tricky techniques it is possible to overcome the preservation and unmasking of those labile antigens involved in signal transduction. Well, by seventheen chapters grouped in five parts Prof. Alexander E. Kalyuzhny is presenting an invaluable technical and methodological source of hints to satisfy our needs: to overcome troubleshottings if we are already in the field or to orientate those entering the field.” (Carlo Albert Redi, European Journal of Histochemistry, July, 2011)