Del i serien Methods in Molecular Biology

Sertoli Cells

Methods and Protocols

Häftad, Engelska, 2019

1 519 kr

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This detailed volume explores the use of Sertoli cells as models in male reproductive biology or as supporters for other cell types, and it answers the need for standardized protocols for their in vitro culture.

Produktinformation

Utgivningsdatum2019-06-06
Mått178 x 254 x 17 mm
Vikt590 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor297
FörlagHumana Press Inc.
ISBN9781493985432

Tillhör följande kategorier

Cellbiologi inom Naturvetenskap och teknik

Establishment of Primary Culture of Sertoli Cells.- Evaluation of the Purity of Sertoli Cell Primary Cultures.- Preparation of Testicular Samples for Histology and Immunohistochemistry.- Rabbit Sertoli Cells: Immunohistochemical Profile from Neonatal to Adult Age.- Identification of Proliferative and Apoptotic Sertoli Cells Using Fluorescence and Confocal Microscopy.- Sertoli Cells Preparation for Co-Immunoprecipitation.- Profiling Signaling Proteins in Sertoli Cells by Co-Immunoprecipitation.- Phagocytosis by Sertoli Cells: Analysis of Main Phagocytosis Steps by Confocal and Electron Microscopy.- A Method for In Vivo Induction and Ultrastructural Detection of Mitophagy in Sertoli Cells.- Assessing Autophagy in Sertoli Cells.- Molecular Mechanisms and Signaling Pathways Involved in the Nutritional Support of Spermatogenesis by Sertoli Cells.- Assessing Sertoli Cells Metabolic Activity.- Proteome Profiling of Sertoli Cells Using a GeLC-MS/MS Strategy.- Gene Silencing of Human Sertoli Cells Utilizing Small Interfering RNAs.- Testicular Cell Selective Ablation Using Diphtheria Toxin Receptor Transgenic Mice.- Regulation of Blood-Testis Barrier (BTB) Dynamics, Role of the Actin, and Microtubule-Based Cytoskeletons.- Monitoring the Integrity of the Blood-Testis Barrier (BTB): An In Vivo Assay.- Computational Methods Involved in Evaluating the Toxicity of the Reproductive Roxicants in Sertoli Cell.- A Stopped-Flow Light Scattering Methodology for Assessing the Osmotic Water Permeability of Whole Sertoli Cells.- Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing.