This book LNICST 622 constitutes the refereed proceedings of the Second EAI International Conference on Security and Privacy in Cyber-Physical Systems and Smart Vehicles, SmartSP 2024, held in New Orleans, LA, USA, during November 7–8, 2024.The 18 full papers were carefully reviewed an selected from 47 submissions. The proceedings focus on Emerging Applications, Hardware and Firmware Security, Adversarial Attacks in Autonomous Systems, Ethics, Privacy, Human-Centric Considerations and Security Techniques for Cyber-Physical Systems.