This book constitutes the refereed post-conference proceedings of the 7th International Conference, on Science of Cyber Security, SciSec 2025, held in Wuhan, China, during August 15-17, 2025. The 19 full papers presented in this volume were carefully reviewed and selected from 66 submissions. The mission of the conference was to foster a deeper understanding of, and to build a solid foundation for, the emerging science of cybersecurity.