RNA Therapeutics

• RNA Modifications: A Mechanism that Modulates Gene Expression.- Quantitative Analysis of RNA Modifications.- Advances in RNA Sensing by the Immune System: Separation of siRNA Unwanted Effects from RNA Interference.- Progress in siRNA Delivery Using Multifunctional Nanoparticles.- Optimized Protocols for siRNA Delivery into Monocytes and Dendritic Cells.- Systemic Delivery of Synthetic siRNAs.- What Are the Key Targeted Delivery Technologies of siRNA Now?.- Using OligoWalk to Identify Efficient siRNA Sequences.- Jet-Injection of Short Hairpin RNA-Encoding Vectors into Tumor Cells.- Short Hairpin RNA (shRNA): Design, Delivery, and Assessment of Gene Knockdown.- Effective Pol III-Expressed Long Hairpin RNAs Targeted to Multiple Unique Sites of HIV-1.- Functional Studies on RNA-Transfected Cell Microarrays.- Transfection Microarrays for High-Throughput Phenotypic Screening of Genes Involved in Cell Migration.- Intron-Mediated RNA Interference, Intronic MicroRNAs, and Applications.- Inhibition of the microRNA Pathway in Zebrafish by siRNA.- Profiling of miRNA Expression and Prediction of Target Genes.- Small RNA Cloning.- In Situ Hybridization Detection of microRNAs.- Detection and Quantitative Analysis of Small RNAs by PCR.- In Vivo Reprogramming of Human Telomerase Reverse Transcriptase (hTERT) by Trans-Splicing Ribozyme to Target Tumor Cells.- Design and Function of Triplex Hairpin Ribozymes.- Catalytic M1GS RNA as an Antiviral Agent in Animals.- Using Live Cells to Generate Aptamers for Cancer Study.- Primer-Free Aptamer Selection Using a Random DNA Library.- Development of TLR7/8 Small RNA Antagonists.- Modulation of Dendritic Cell Maturation and Function by siRNA-Bearing 5?-Triphosphate.- Immunotherapy of Cancer with Dendritic Cells Loaded with Tumor Antigens andActivated Through mRNA Electroporation.- Non-MHC-Dependent Redirected T Cells Against Tumor Cells.- Overcoming Self-Tolerance to Tumour Cells.- Recent Advances in Hematopoietic Stem Cell Transplantation and Perspectives of RNAi Applications.

From the reviews: "Give us an update statement of the present day possible use of RNA to treat several diseases are well presented thanks to a 30 chapters walk in the RNA world. ... Clearly enough, the book is of great interest to the entire scientific community, no matter if basic researchers, teachers or clinicians!" (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)