RNA Editing

Jonatha Gott works in the Center for RNA Molecular Biology at Case Western Reserve University, Cleveland, Ohio, USA.

• Trypanosome U insertion/deletion.Chapter 1: Isolation and Compositional Analysis of Trypanosomatid Editosomes. Chapter 2: U insertion/deletion editing activities.Chapter 3: RNA Editing Uridylyltransferases of Trypanosomes. Chapter 4: Isolation of RNA binding proteins involved in insertion/deletion editingChapter 5: RNA-protein Interactions in Assembled Editing Complexes in Trypanosomes.Chapter 6: Strategies of Kinetoplastid Cryptogene Discovery and Analysis.Physarum insertion editing.Chapter 7: RNA editing in Physarum mitochondria: assays and biochemical approaches.Chapter 8: Computational approaches to insertional RNA editing. Chapter 9: Evolution of RNA Editing Sites in the Mitochondrial Small Subunit rRNA of the Myxomycota.5' deletion/insertion editing of tRNAs. Chapter 10: Analysis of 5´- or 3´-Terminal tRNA Editing: Mitochondrial 5´ tRNA Editing in Acanthamoeba castellanii as the Exemplar.A to I editing.Chapter 11: Comparative genomic and bioinformatic approaches for the identification of new adenosine to inosine substrates.Chapter 12: Genetic approaches to studing adenosine to inosine RNA editing. Chapter 13: A method to find sites of selective adenosine deamination.Chapter 14 : Purification and assay of ADAR activity.Chapter 15: Large Scale Overexpression and Purification of ADARs from S. cerevisiae for Biophysical and Biochemical Studies.Chapter 16: Mouse models to elucidate the functional roles of A-to-I editing.Chapter 17: Probing Adenosine to Inosine Editing Reactions using RNA containing Nucleoside Analogs.Nuclear C to U editing.Chapter 18: Measuring Editing Activity and Identifying C to U mRNA Editing Factors in Cells and Biochemical Isolates.Chapter 19: Mouse Models as Tools to Explore C to U RNA Editing.Editing in plant organellesChapter 20: RNA editing in plant mitochondria: assays and biochemical approaches. Chapter 21: Assay of Editing of Exogenous RNAs in Chloroplast Extracts of Arabidopsis, Maize, Pea, and Tobacco.Chapter 22: In organello gene expression and RNA editing studies by electroporation-mediated transformation of isolated plant mitochondria.Chapter 23: Transformation of the Plastid Genome to Study RNA Editing.