Del 2869 i serien Methods in Molecular Biology

Rice

Methods and Protocols

Inbunden, Engelska, 2024

3 019 kr

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Chapters detail sample preparation, temporal and spatial analysis of RNA, protein expression, transformation and precise genome editing, scalable transcriptome analysis, epigenome analysis, and gene regulatory network analysis.

Produktinformation

Utgivningsdatum2024-11-06
Mått178 x 254 x 17 mm
Vikt598 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor195
FörlagSpringer-Verlag New York Inc.
ISBN9781071642030

Tillhör följande kategorier

Biokemisk teknik inom Naturvetenskap och teknik
Botanik inom Naturvetenskap och teknik
Växtböcker inom Djur och Natur
Agronomi inom Naturvetenskap och teknik

Hydroponic culture of rice seedlings for stress response assay.- Total RNA extraction from rice vegetative tissues using magnetic beads.- Total RNA extraction from starchy rice endosperm.- RNA extraction from rice immature embryo using laser capture microdissection.- Using immunoblotting for detecting of a circadian clock component, Os−GI, in rice leaves.- Preparation and Sectioning of Paraffin-Embedded Tissue for Histology and Histochemistry.- Spatial Analysis of Gene Expression by In Situ Hybridization.- Purification of Anti-OSH1 IgG and Immunohistochemical Staining of Rice Shoot Apical Meristem.- Agrobacterium-mediated transformation and targeted mutagenesis using SpCas12f in rice.- Gene targeting via CRISPR/Cas9-mediated DNA double-strand break induction in rice.- Precise base substitution using CRISPR/Cas-mediated base editor in rice.- DeLTa-seq: High-throughput targeted RNA-seq of rice leaves without RNA purification.- Lasy-seq: a high-throughput 3′ RNA-seq method for large-scale transcriptome profiling in rice.- Profiling of 24nt phasiRNAs in rice anther by massively parallel sequencing and stem-loop reverse transcription PCR.- Expression and purification of pAG-MNase for CUT&RUN.- Chromatin immunoprecipitation from shoot apices and young panicles of rice.- In vitro Genome-wide identification of transcription factor binding sites.- High-efficient transient gene transfection for transcriptional activity assay using rice protoplasts on large-scale platform.