Del 550 - Methods in Enzymology

Riboswitches as Targets and Tools

Inbunden, Engelska, 2015

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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in riboswitches as targets and tools and contains sections on such topics as constructing and optimizing artificial riboswitches, live cell imaging and intracellular sensors with artificial riboswitches, conditional control of gene expression with artificial riboswitches, using artificial riboswitches for protein evolution and pathway optimization, and anti-riboswitch drug screens.

Continues the legacy of this premier serial with quality chapters authored by leaders in the field
Covers research methods in riboswitches as targets and tools
Contains sections on such topics as constructing and optimizing artificial riboswitches, synthetic biology: live cell imaging and intracellular sensors with artificial riboswitches, synthetic biology: conditional control of gene expression with artificial riboswitches, synthetic biology: using artificial riboswitches for protein evolution and pathway optimization, anti-riboswitches drug screens

Produktinformation

Utgivningsdatum2015-01-13
Mått152 x 229 x undefined mm
Vikt880 g
FormatInbunden
SpråkEngelska
SerieMethods in Enzymology
Antal sidor444
FörlagElsevier Science
MedarbetareBurke-Aguero,DonaldH.
ISBN9780128011232

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1. Design of Transcription Regulating RiboswitchesSven Findei, Manja Wachsmuth, Mario M¨orl, and PeterF. Stadler2. Ligand-Dependent Exponential Amplification of Self-Replicating RNA EnzymesCharles Olea, Jr. and Gerald F. Joyce3. Design of Modular “Plug-and-Play Expression Platforms Derived From Natural Riboswitches for Engineering Novel Genetically Encodable RNA Regulatory DevicesJeremiah J. Trausch and Robert Batey4. Integrating and Amplifying Signal from Riboswitch BiosensorsMichael S. Goodson, Svetlana V. Harbaugh, Yaroslav G. Chushak and Nancy Kelley-Loughnane5. Simple identification of two causes of noise in an aptazyme system by monitoring cell-free transcription. Norikazu Ichihashi, Shungo Kobori, Tetsuya Yomo6. Engineering of ribosomal shunt-modulating eukaryotic ON riboswitches by using a cell-free translation systemAtsushi Ogawa7. Chemo-enzymatic Synthesis of Selectively 13C/15N-labeled RNA for NMR Structural and Dynamics StudiesLuigi J. Alvarado, Andrew P. Longhini, Regan LeBlanc, Bin Chen, Christoph Kreutz and T. Kwaku Dayie8. In Vitro Analysis of Riboswitch-Spinach Aptamer Fusions as Metabolite-Sensing Fluorescent BiosensorsColleen A. Kellenberger and Ming C. Hammond9. Assaying Break and Nick-Induced Homologous Recombination in Mammalian Cells Using the DR-GFP Reporter and Cas9 Nucleases.Lianne E.M. Vriend , Maria Jasin and Przemek M. Krawczyk10. Adapting CRISPR/Cas9 for Functional Genomics ScreensAbba Malina , Alexandra Katigbak , Regina Cencic , Rayelle Itoua Maïga , Francis Robert, Hisashi Miura and Jerry Pelletier11. The Icrispr Platform for Rapid Genome Editing in Human Pluripotent Stem CellsZengrong Zhu, Federico González and Danwei Huangfu12. Using Riboswitches to Regulate Gene Expression and Define Gene Function in MycobacteriaErik Van Vlack and Jessica C. Seeliger13. Controlling Expression of Genes in the Unicellular Alga Chlamydomonas Reinhardtii With a Vitamin-Repressible RiboswitchSilvia Ramundo and Jean-David Rochaix14. Conditional Control of Gene Expression by Synthetic Riboswitches in Streptomyces CoelicolorMartin M. Rudolph, Michael-Paul Vockenhuber und Beatrix Suess15. Engineering of Ribozyme-Based Aminoglycoside Switches of Gene Expression by in Vivo Genetic Selection in Saccharomyces CerevisiaeBenedikt Klauser, Charlotte Rehm and Jörg S. Hartig16. Kinetic Folding Design of Aptazyme-Regulated Expression Devices (aREDs) as Riboswitches for Metabolic EngineeringDavid Sparkman-Yager, Rodrigo A Correa-Rojas and James M Carothers17. Riboselector: Riboswitch-Based Synthetic Selection Device to Expedite Evolution of Metabolite-Producing MicroorganismsSungho Jang, Jina Yang, Sang Woo Seo and Gyoo Yeol Jung18. Fluorescence Assays for Monitoring RNA-Ligand Interactions and Riboswitch-Targeted Drug Discovery ScreeningJ. Liu, C. Zeng, S. Zhou, J.A. Means and J.V. Hines19. Monitoring Ribosomal Frameshifting as a Platform to Screen Anti-Riboswitch Drug CandidatesChien-Hung Yu and René C. L. Olsthoorn