Receptor Purification is a unique practical two-volume guide to the purification of most receptor types including receptors for neurotransmitters polypeptide hormones steroid hormones and a variety of other ligands involved in many biological processes. Today's methods of receptor purification have evolved from years of research on the mechanisms of receptor actions where they were needed to discover receptor properties and ultimately for use in cloning. Receptor Purification provides a comprehensive source of techniques to enable the purification of cloned receptors as well as a handy reference on purification of receptors from cells and tissues in a physiological context. Designed to give investigators the best possible selection of approaches to a purification problem alternative methods are provided where applicable. In addition Receptor Purification features chapters on specific agents-such as covalent binding ligands-that will greatly simplify some purification processes. Many of the leading laboratories whose work has led to the purification of a wide variety of receptors are represented in the contributions to these two volumes.In some chapters information has been added on the applications of purified receptors while others focus on the cloning of the receptors. The purification of receptors is group-based on functional ligands. The first volume is devoted to the purification of receptors that bind neurotransmitters and a wide variety of polypeptide hormones. Volume 2 covers the steroid receptors and associated proteins such as vitamin D-3 and retinoic acid receptors. Other polypeptide receptors such as those involved in