Quantum Dots captures many diverse applications enabling utility in biological detection. Organized into five parts, the first two parts cover the use of QDs in imaging fixed and living cells (and tissues). Protocols are included for using QDs in routine (protein and structural cellular labeling), as well as enabling (single receptor trafficking, clinical pathology, correlative microscopy) applications. Part 3 shows early efforts aimed at using QDs in live animals. The final 2 parts demonstrate the versatility of QD technology in existing assay technology.

Produktinformation

Utgivningsdatum2007-02-02
Mått152 x 229 x 22 mm
Vikt626 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor257
Upplaga2007
FörlagHumana Press Inc.
ISBN9781588295620

Tillhör följande kategorier

Biokemisk teknik inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik

Imaging of Fixed Cells and Tissues.- Immunofluorescent Labeling of Proteins in Cultured Cells With Quantum Dot Secondary Antibody Conjugates.- Immunohistochemical Detection With Quantum Dots.- Quantum Dots for Multicolor Tumor Pathology and Multispectral Imaging.- Light and Electron Microscopic Localization of Multiple Proteins Using Quantum Dots.- Human Metaphase Chromosome FISH Using Quantum Dot Conjugates.- Imaging of Live Cells.- Biotin-Ligand Complexes With Streptavidin Quantum Dots for In Vivo Cell Labeling of Membrane Receptors.- Single Quantum Dot Tracking of Membrane Receptors.- Optical Monitoring of Single Cells Using Quantum Dots.- Peptide-Mediated Intracellular Delivery of Quantum Dots.- Multiple Cell Lines Using Quantum Dots.- Measuring Cell Motility Using Quantum Dot Probes.- Imaging of Live Animals.- Quantum Dots for In Vivo Molecular and Cellular Imaging.- Sentinel Lymph Node Mapping With Type-II Quantum Dots.- Macrophage-Mediated Colocalization of Quantum Dots in Experimental Glioma.- Flow Cytometry Applications.- Application of Quantum Dots to Multicolor Flow Cytometry.- Quantum Dots in Flow Cytometry.- Biochemical Applications.- Luminescent Biocompatible Quantum Dots.- Fluorescence-Based Analysis of Cellular Protein Lysate Arrays Using Quantum Dots.- Application of Quantum Dots to Multicolor Microarray Experiments.

From the reviews: "...provides an excellent reference for protocols that can be utlized repeatedly to yield reliable performance from quantum dot-enabled applications." -Anticancer Research 27, May 2007 "Bruschez and Holtz have compiled an extremely useful set of applications and protocols in Quantum Dots, Applications in Biology. The protocols, written by the many leaders in QD imaging, cover a wide range of applications and provide detailed methodology for each application supported by excellent illustrations. This book certainly belongs on the shelf of anyone interested in biological imaging. ... in summary, this illustrative compilation of well-written protocols on QD is a must have for both the expert and novice interested in biological imaging." (Neel Ghosh, ChemBioChem, Issue 9, 2008)