Protoplast Technology

Methods and Protocols

Inbunden, Engelska, 2022

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The book begins with chapters that focus on basic protoplast techniques and their utilities, such as protocols on protoplast isolation, transfection, and regeneration, as well as examples of how to use protoplasts for genome editing and gene function analysis in a number of major crop or model plant species.

Produktinformation

Utgivningsdatum2022-03-09
Mått178 x 254 x 21 mm
Vikt722 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor263
FörlagSpringer-Verlag New York Inc.
ISBN9781071621639

Tillhör följande kategorier

Botanik inom Naturvetenskap och teknik
Växtböcker inom Djur och Natur
Biovetenskap inom Naturvetenskap och teknik

Physcomitrium patens Protoplasting and Protoplast Transfection.- Protoplast Isolation and Transfection in the Single-Cell C4 Species Bienertia sinuspersici.- Protoplast for Gene Functional Analysis in Arabidopsis.- Application of Protoplast Regeneration to CRISPR/Cas9 Mutagenesis in Nicotiana tabacum.- CRISPR DNA- and RNP-Mediated Genome Editing via Nicotiana benthamiana Protoplast Transformation and Regeneration.- Protoplast Isolation and Transfection in Rice.- Protoplast Isolation and Transfection in Maize.- Isolation and Transfection of Maize Endosperm Protoplasts.- Protoplast Isolation from Undifferentiated Maize Seedling Shoot Tissue.- Protoplast Isolation and Transfection in Wheat.- Protoplast Isolation, Transformation, and Regeneration for Forage and Turf Grasses.- Efficient Protoplast Isolation and DNA Transfection for Winter Oilseed Crops, Pennycress (Thlaspi arvense) and Camelina (Camelina sativa).- Protoplast Isolation,Transfection, and Gene Editing for Soybean (Glycine max).- Protoplast Isolation and Transformation in Oil Palm.- Protoplast Preparation and Fluorescence-Activated Cell Sorting for the Evaluation of Targeted Mutagenesis in Plant Cells.- Characterization of Programmable Transcription Activators in the Model Monocot Setaria viridis via Protoplast Transfection.- High-Throughput Transfection and Analysis of Soybean (Glycine max) Protoplasts.