Protocols for Adult Stem Cells

• In Situ Analysis of Cell Populations: Long-Term Label-Retaining Cells.- Limiting-Dilution Transplantation Assays in Mammary Stem Cell Studies.- Use of Stem Cell Markers in Dissociated Mammary Populations.- Isolation of Adult Hippocampal Neural Progenitors.- In Vitro Culture and Analysis of Adult Hippocampal Neural Progenitors.- In Vivo Analysis of Engrafted Adult Hippocampal Neural Progenitors.- Viral Packaging and Transduction of Adult Hippocampal Neural Progenitors.- Culture of Bone Marrow Mesenchymal Stem Cells on Engineered Matrix.- Preparation and Analysis of Endothelial Progenitor Cells Associated with Angiogenesis.- Preparation of Adult Muscle Fiber-Associated Stem/Precursor Cells.- Immuno-Analysis and FACS Sorting of Adult Muscle Fiber-Associated Stem/Precursor Cells.- Altered Expression of NFkB in Ex Vivo Differentiated Dendritic Cells from the Aged Subjects: Implications in Immunotherapy.- Preparation of Collagen-Coated Gels that Maximize In Vitro Myogenesis of Stem Cells by Matching the Lateral Elasticity of In Vivo Muscle.

From the reviews:“This volume in the popular Methods in Molecular Biology series provides a snapshot of research techniques and protocols for identifying and culturing these amazing tissue-specific stem cells. … The book is written for basic science and clinical researchers interested in stem cell biology, as well as mechanisms of carcinogenesis and malignancy. It also will be valued by biomedical scientists and clinicians interested in cytotechnology and biotechnology. … The topic is fascinating and clinically relevant. I enjoyed learning about antigen-priming dendritic cells.” (Bruce A. Fenderson, Doody’s Review Service, August, 2010)