Proteomic Biology Using LC/MS

Nobuhiro Takahashi, PhD, is a Professor of Applied Biological Science in the Department of Agriculture at Tokyo University of Agriculture and Technology.Toshiaki Isobe, PhD, is a Professor of Biochemistry in the Department of Chemistry at Tokyo Metropolitan University.

• Introduction vii1 Overview of Proteomics 11-1 What Is Proteomics?, 11-2 Establishment of Proteomics Using Mass Spectrometry, 31-2-1 Protein Separations by Two-Dimensional Electrophoresis, 31-2-2 Development of the Technologies for Protein Identification, 51-2-3 Protein Identification Based on Gel Separation and Mass Spectrometry, 61-3 Strategies for Characterizing Proteomes and Understanding Proteome Function, 151-3-1 Modification-Specific Proteomics, 171-3-2 Activity-Based Protein Profiling/Enzyme Substrate Proteomics, 311-3-3 Subcellular (Organellar) Proteomics, 411-3-4 Machinery or Complex Interaction Proteomics, 451-3-5 Dynamic Proteomics, 462 Proteomic Tools for Analysis of Cellular Dynamics 632-1 LC-Based Proteomics Technologies, 632-1-1 LC-MS system for Peptide Separation and Identification, 712-1-2 Application of LC-MS Methods to Functional Proteomics, 1012-2 Development of Quantitative Proteomics, 1312-2-1 Isotope Labeling for Quantitative Analysis Using MS, 1322-2-2 Quantification Strategy for LC-MS Analysis of Isotope-Labeled Peptide Mixture and Software for ComputerAnalysis, 1402-2-3 Label-Free Quantification Software, 1452-2-4 Absolute Quantification, 1473 Dynamics of Functional Cellular Machinery: From Statics to Dynamics in Proteomic Biology 1673-1 Dynamic Analysis of Cellular Function, 1673-1-1 Strategy for Dynamic Analysis of Cellular Machinery (Multiprotein Complexes), 1683-1-2 Methods for the Isolation of a Cellular Machine/Multiprotein Complex, 1743-1-3 Cellular Machinery (Multiprotein Complex), 1963-2 Dynamics of Ribosome Biogenesis, 1983-2-1 Snapshot Analysis of Preribosomal Particles in Yeast, 2013-2-2 Snapshot Analysis of Preribosomal Particles in Mammals, 2073-2-3 Quantitative (Dynamic) Analysis Using Isotope-Labeled Reagents, 2243-2-4 Outline of Human/Mammalian Ribosome Biogenesis, 2313-3 Dynamic Analysis of Subcellular Structures, 2323-3-1 Proteome Dynamics of the Nucleolus, 233References, 236Index 249

"The book covers the topic of proteomics and mass spectrometry analysis well and does so at a reasonably advanced level. This volume would be suitable for advanced graduate students and for nonexpert scientists to learn about the application of mass spectrometry techniques for proteomic studies." (Quarterly Review of Biology, December 2008)