JAN-CHRISTER JANSON is Professor Emeritus at the Department of Physical and Analytical Chemistry, Uppsala University, Uppsala, Sweden.

• Preface to The Third Edition viiPreface to The Second Edition ixPreface to The First Edition xiContributors xiiiPart I Introduction 11 Introduction to Protein Purification 3Bo Ersson, Lars Rydén, and Jan-Christer JansonPart II Chromatography 232 Introduction to Chromatography 25Jan-Christer Janson and Jan Åke Jönsson3 Gel Filtration: Size Exclusion Chromatography 51Lars Hagel4 Ion Exchange Chromatography 93Evert Karlsson and Irwin Hirsh5 High-Resolution Reversed-Phase Chromatography of Proteins 135Sylvia Winkel Pettersson6 Hydrophobic Interaction Chromatography 165Kjell-Ove Eriksson and Makonnen Belew7 Immobilized Metal Ion Affinity Chromatography 183Lennart Kågedal8 Covalent Chromatography 203Francisco Batista-Viera, Lars Rydén, and Jan Carlsson9 Affinity Chromatography 221Francisco Batista-Viera, Jan-Christer Janson, and Jan Carlsson10 Affinity Ligands from Chemical Combinatorial Libraries 259Enrique Carredano and Herbert Baumann11 Affinity Ligands from Biological Combinatorial Libraries 269Per- Åke NygrenPart III Other Separation Methods and Related Techniques 27912 Membrane Separations 281Joachim K. Walter, Zuwei Jin, Maik W. Jornitz, and Uwe Gottschalk13 Refolding of Inclusion Body Proteins from E. coli 319Zhiguo Su, Diannan Lu, and Zheng Liu14 Purification of PEGylated Proteins 339Conan J. Fee and James M. Van AlstinePart IV Electrophoresis 36315 Electrophoresis in Gels 365Reiner Westermeier16 Conventional Isoelectric Focusing in Gel Slabs and Capillaries and Immobilized pH Gradients 379Pier Giorgio Righetti, Elisa Fasoli, and Sabina Carla Righetti17 Two-Dimensional Electrophoresis in Proteomics 411Reiner Westermeier and Angelika Görg18 Protein Elution and Blotting Techniques 441Reiner Westermeier19 Capillary Electrophoretic Separations 451Wolfgang ThormannPart V Separation Method Optimization 48720 High Throughput Screening Techniques in Protein Purification 489Karol M. Lacki and Eggert BrekkanIndex 507

JAN-CHRISTER JANSON is Professor Emeritus at the Department of Physical and Analytical Chemistry, Uppsala University, Uppsala, Sweden.

• Preface to The Third Edition viiPreface to The Second Edition ixPreface to The First Edition xiContributors xiiiPart I Introduction 11 Introduction to Protein Purification 3Bo Ersson, Lars Rydén, and Jan-Christer JansonPart II Chromatography 232 Introduction to Chromatography 25Jan-Christer Janson and Jan Åke Jönsson3 Gel Filtration: Size Exclusion Chromatography 51Lars Hagel4 Ion Exchange Chromatography 93Evert Karlsson and Irwin Hirsh5 High-Resolution Reversed-Phase Chromatography of Proteins 135Sylvia Winkel Pettersson6 Hydrophobic Interaction Chromatography 165Kjell-Ove Eriksson and Makonnen Belew7 Immobilized Metal Ion Affinity Chromatography 183Lennart Kågedal8 Covalent Chromatography 203Francisco Batista-Viera, Lars Rydén, and Jan Carlsson9 Affinity Chromatography 221Francisco Batista-Viera, Jan-Christer Janson, and Jan Carlsson10 Affinity Ligands from Chemical Combinatorial Libraries 259Enrique Carredano and Herbert Baumann11 Affinity Ligands from Biological Combinatorial Libraries 269Per- Åke NygrenPart III Other Separation Methods and Related Techniques 27912 Membrane Separations 281Joachim K. Walter, Zuwei Jin, Maik W. Jornitz, and Uwe Gottschalk13 Refolding of Inclusion Body Proteins from E. coli 319Zhiguo Su, Diannan Lu, and Zheng Liu14 Purification of PEGylated Proteins 339Conan J. Fee and James M. Van AlstinePart IV Electrophoresis 36315 Electrophoresis in Gels 365Reiner Westermeier16 Conventional Isoelectric Focusing in Gel Slabs and Capillaries and Immobilized pH Gradients 379Pier Giorgio Righetti, Elisa Fasoli, and Sabina Carla Righetti17 Two-Dimensional Electrophoresis in Proteomics 411Reiner Westermeier and Angelika Görg18 Protein Elution and Blotting Techniques 441Reiner Westermeier19 Capillary Electrophoretic Separations 451Wolfgang ThormannPart V Separation Method Optimization 48720 High Throughput Screening Techniques in Protein Purification 489Karol M. Lacki and Eggert BrekkanIndex 507

JAN-CHRISTER JANSON is Professor Emeritus at the Department of Physical and Analytical Chemistry, Uppsala University, Uppsala, Sweden.

• Preface to The Third Edition viiPreface to The Second Edition ixPreface to The First Edition xiContributors xiiiPart I Introduction 11 Introduction to Protein Purification 3Bo Ersson, Lars Rydén, and Jan-Christer JansonPart II Chromatography 232 Introduction to Chromatography 25Jan-Christer Janson and Jan Åke Jönsson3 Gel Filtration: Size Exclusion Chromatography 51Lars Hagel4 Ion Exchange Chromatography 93Evert Karlsson and Irwin Hirsh5 High-Resolution Reversed-Phase Chromatography of Proteins 135Sylvia Winkel Pettersson6 Hydrophobic Interaction Chromatography 165Kjell-Ove Eriksson and Makonnen Belew7 Immobilized Metal Ion Affinity Chromatography 183Lennart Kågedal8 Covalent Chromatography 203Francisco Batista-Viera, Lars Rydén, and Jan Carlsson9 Affinity Chromatography 221Francisco Batista-Viera, Jan-Christer Janson, and Jan Carlsson10 Affinity Ligands from Chemical Combinatorial Libraries 259Enrique Carredano and Herbert Baumann11 Affinity Ligands from Biological Combinatorial Libraries 269Per- Åke NygrenPart III Other Separation Methods and Related Techniques 27912 Membrane Separations 281Joachim K. Walter, Zuwei Jin, Maik W. Jornitz, and Uwe Gottschalk13 Refolding of Inclusion Body Proteins from E. coli 319Zhiguo Su, Diannan Lu, and Zheng Liu14 Purification of PEGylated Proteins 339Conan J. Fee and James M. Van AlstinePart IV Electrophoresis 36315 Electrophoresis in Gels 365Reiner Westermeier16 Conventional Isoelectric Focusing in Gel Slabs and Capillaries and Immobilized pH Gradients 379Pier Giorgio Righetti, Elisa Fasoli, and Sabina Carla Righetti17 Two-Dimensional Electrophoresis in Proteomics 411Reiner Westermeier and Angelika Görg18 Protein Elution and Blotting Techniques 441Reiner Westermeier19 Capillary Electrophoretic Separations 451Wolfgang ThormannPart V Separation Method Optimization 48720 High Throughput Screening Techniques in Protein Purification 489Karol M. Lacki and Eggert BrekkanIndex 507