Protein-Protein Interactions

Methods and Protocols

Häftad, Engelska, 2024

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This detailed volume provides a comprehensive collection of classic and cutting-edge methods and techniques in mapping protein-protein interactions. In addition, the book also explores in silico methods including sequence-, structure-, and phylogenetic profile-based approaches as well as gene expression and machine learning methods.

Produktinformation

Utgivningsdatum2024-07-15
Mått178 x 254 x 28 mm
Vikt938 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor486
FörlagSpringer-Verlag New York Inc.
ISBN9781071633298

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Biokemi inom Naturvetenskap och teknik

Yeast-Two-Hybrid Technique to Identify Protein-Protein Interactions.- Cytotrap: An Innovative Approach for Protein-Protein Interaction Studies for Cytoplasmic Proteins.- Analyzing Protein-Protein Interactions Using the Split-Ubiquitin System.- Detection of Protein–Protein Interactions Utilizing the Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System.- Preparation and Utilization of a Versatile GFP-Protein Trap-Like System for Protein Complex Immunoprecipitation in Plants.- Tandem Affinity Purification (TAP) of Interacting Prey Proteins with FLAG- and HA-Tagged Bait Proteins.- Affinity Purification-Mass Spectroscopy (AP-MS) and Co-Immunoprecipitation (Co-IP) Technique to Study Protein-Protein Interactions.- Co-Immunoprecipitation-Based Identification of Effector-Host Protein Interactions from Pathogen-Infected Plant Tissue.- Co-Immunoprecipitation for Assessing Protein-Protein Interactions in Agrobacterium-Mediated Transient Expression System in Nicotiana benthamiana.-Detection of Protein-Protein Interactions Using Glutathione-S-Transferase (GST) Pull-Down Assay Technique.- Bimolecular Fluorescence Complementation (BiFC) Assay to Visualize Protein-Protein Interactions in Living Cells.- Detecting Protein-Protein Interactions Using Bimolecular Fluorescence Complementation (BiFC) and Luciferase Complementation Assays (LCA).- Forster Resonance Energy Transfer (FRET) to Visualize Protein-Protein Interactions in the Plant Cell.- Dynamic Proximity Tagging in Living Plant Cells with Pupylation-Based Interaction Tagging.- Characterization of Small Molecule-Protein Interactions Using SPR Method.- High-Throughput Protein-Protein Interactions Screening Using Pool-Based Liquid Yeast Two-Hybrid Pipeline.- Dynamic Enrichment for Evaluation of Protein Networks (DEEPN): A High Throughput Yeast Two-Hybrid (Y2H) Protocol to Evaluate Networks.- An Interactome Assay for Detecting Interactions between Extracellular Domains of Receptor Kinases.- Next-Generation YeastTwo-Hybrid Screening to Discover Protein-Protein Interactions.- Bioinformatic Analysis of Yeast Two-Hybrid Next-Generation Interaction Screen Data.- Discovering Protein-Protein Interactions Using Co-Fractionation-Mass Spectrometry with Label-Free Quantitation.- Protein-Protein Interaction Network Mapping by Affinity Purification Cross-Linking (AP-CXL-MS) Proteomics.- Protein Interaction Screen on a Peptide Matrix (PrISMa).- Analyzing Protein Interactions by MAC-Tag Approaches.- Identification and Quantification of Affinity-Purified Proteins with MaxQuant, Followed by the Discrimination of Nonspecific Interactions with the CRAPome Interface.- Cataloguing Protein Complexes In Planta Using TurboID-Catalyzed Proximity Labeling.- A Data-Driven Signaling Network Inference Approach for Phosphoproteomics.- Pairwise and Multi-Chain Protein Docking Enhanced Using LZerD Web Server.- Predicting Protein Interaction Sites Using PITHIA.- Using PlaPPISite to Predict and Analyze Plant Protein-ProteinInteraction Sites.- Machine Learning Methods for Virus-Host Protein-Protein Interaction Prediction.- Protein-Protein Interaction Network Exploration Using Cytoscape.- Search, Retrieve, Visualize, and Analyze Protein-Protein Interactions from Multiple Databases: A Guide for Experimental Biologists.- Centrality Analysis of Protein-Protein Interaction Networks Using R.- Protein-Protein Interaction Network Analysis Using NetworkX.- Building Protein-Protein Interaction Graph Database Using Neo4j.