Protein-Ligand Interactions

Methods and Applications

Häftad, Engelska, 2021

AvTina Daviter,Christopher M. Johnson,Stephen H. McLaughlin,Mark A. Williams

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This third edition provides new and updated chapters detailing a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins with their natural ligands, drugs or other binding partners.

Produktinformation

Utgivningsdatum2021-04-21
Mått178 x 254 x 28 mm
Vikt938 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor492
Upplaga3
FörlagSpringer-Verlag New York Inc.
ISBN9781071611999

Tillhör följande kategorier

Kemi inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik
Farmakologi inom Medicin

Assessing and Improving Protein Sample Quality.- A Familiar Protein-Ligand Interaction Revisited with Multiple Methods.- Interactions of a Signal Transduction Protein Investigated by Fluorescence Stopped-Flow Kinetics.- Kinetic Methods of Deducing Binding Mechanisms Involving Intrinsically Disordered Proteins.- Isothermal Titration Calorimetry.- Measuring the Kd of Protein-Ligand Interactions Using Microscale Thermophoresis.- Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D); Preparing Functionalized Lipid Layers for the Study of Complex Protein-Ligand Interactions.- Indirect Detection of Ligand Binding by Thermal Melt Analysis.- The Use of Acoustic Mist Ionization Mass Spectrometry for High-throughput Screening.- Ligand Discovery – High Through-put Binding: Fluorescence Polarization (Anisotropy).- Fragment Screening by NMR.- A Quick Primer in Fluorescence-based Equilibrium and Pre-Steady State Methods for Determining Protein-Nucleotide Affinities.- Measurement of Nucleotide Hydrolysis using Fluorescent Biosensors for Phosphate.- Gel-based Analysis of Protein-Nucleic Acid Interactions.- Biophysical Studies of the Binding of Viral RNA with the 80S Ribosome using SwitchSENSE.- Biolayer Interferometry: Protein-RNA Interactions.- Analysis of Protein-DNA Interactions Using Surface Plasmon Resonance and a ReDCaT Chip.- Characterization of Protein Nucleic Acid complexes by Size-Exclusion Chromatography Coupled with Light Scattering, Absorbance and Refractive Index Detectors.- Analytical Ultracentrifugation for Analysis of Protein Nucleic Acid Interactions.- Studying RNA-Protein Complexes Using X-ray Crystallography.- Flow Linear Dichroism of Protein-membrane Systems.- Probing Protein-Membrane Interactions and Dynamics Using Hydrogen-Deuterium eXchange Mass Spectrometry (HDX-MS).