Protein Engineering: A Practical Approach

• 1. Methods of structural analysis of proteins - part 1 - protein crystallography ; 2. Methods of structural analysis of proteins - part 2 - nuclear magnetic resonance ; 3. Molecular sequence data- bases and their uses ; 4. Design of protein structures ; 5. From protein sequence to structure ; 6. The use of theoretical methods in protein engineering ; 7. Principles of protein stability - part 1 - reversible unfolding of proteins: kinetic and thermodynamic analysis ; 8. Principles of protein stability - part 2 - enhanced folding and stabilization of proteins by suppression of aggregation in vitro and in vivo ; 9. Principles of protein turnover: possible manipulations ; 10. Chemical approaches to expression of proteins ; 11. Protein engineering of antibody combining sites ; 12. Selection of variants of antibodies and other protein molecules using display on the surface of bacteriophage fd ; 13. Expression of proteins in prokaryotic systems - principles and 'case' studies ; 14. Expression of proteins in yeast ; 15. Genetic engineering of the processing and secretory pathway of an inflammatory cytokine in mammalian cells: a case study ; 16. Industrial applications of protein engineering and scale-up of protein production: l-antitrypsin, a case study ; Appendix

'Protein Engineering: A Practical Approach is the first reference book in this network of molecular biology, structure of proteins and biochemistry. It combines theretical background and detailed procedures; both together enable researchers in this field to plan and carry out experiments for their own projects. This book should therefore serve as the standard text for students and scientists in the field of protein engineering.'Arzneimittel-Forschung/Drug Research Issue 12/93