Del 108 i serien Practical Approach Series

Protein Engineering: A Practical Approach

Häftad, Engelska, 1992

1 999 kr

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Protein engineering is the rational modification or redesign of proteins using genetic engineering. Thus, it is now possible to modify enzyme specifities, remodel antibodies, and redesign many multi-domain proteins for therapeutic purposes. While the procedures for the introduction of mutations have become routine, predicitng and understanding the effects of these mutations can be complicated. This volume provides a comprehensive guide to the methods used at every stage of the engineering process, from the choice of mutation strategy, through protein stability studies, to critical evaluations of mammalian, yeast, and bacterial host expression systems. Protein Engineering: A Practical Approach is the first practical guide to this fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry. It combines a thorough theoretical foundation with detailed protocols and will be invaluable to all research workers in the area, from graduate students to senior investigators.

Produktinformation

Utgivningsdatum1992-12-10
Mått156 x 234 x 23 mm
Vikt646 g
FormatHäftad
SpråkEngelska
SeriePractical Approach Series
Antal sidor424
FörlagIRL Press
ISBN9780199631384

Tillhör följande kategorier

Biokemisk teknik inom Naturvetenskap och teknik
Biokemi inom Naturvetenskap och teknik

1. Methods of structural analysis of proteins - part 1 - protein crystallography ; 2. Methods of structural analysis of proteins - part 2 - nuclear magnetic resonance ; 3. Molecular sequence data- bases and their uses ; 4. Design of protein structures ; 5. From protein sequence to structure ; 6. The use of theoretical methods in protein engineering ; 7. Principles of protein stability - part 1 - reversible unfolding of proteins: kinetic and thermodynamic analysis ; 8. Principles of protein stability - part 2 - enhanced folding and stabilization of proteins by suppression of aggregation in vitro and in vivo ; 9. Principles of protein turnover: possible manipulations ; 10. Chemical approaches to expression of proteins ; 11. Protein engineering of antibody combining sites ; 12. Selection of variants of antibodies and other protein molecules using display on the surface of bacteriophage fd ; 13. Expression of proteins in prokaryotic systems - principles and 'case' studies ; 14. Expression of proteins in yeast ; 15. Genetic engineering of the processing and secretory pathway of an inflammatory cytokine in mammalian cells: a case study ; 16. Industrial applications of protein engineering and scale-up of protein production: l-antitrypsin, a case study ; Appendix

'Protein Engineering: A Practical Approach is the first reference book in this network of molecular biology, structure of proteins and biochemistry. It combines theretical background and detailed procedures; both together enable researchers in this field to plan and carry out experiments for their own projects. This book should therefore serve as the standard text for students and scientists in the field of protein engineering.'Arzneimittel-Forschung/Drug Research Issue 12/93