SILVIA MARIA DOGLIA, PhD, is Professor of Physics at the University of Milano-Bicocca, Italy. She received her Laurea degree in Physics at the University of Milano. She has been Staff Research Scientist of the Italian National Research Council; Visiting Scientist at the University of Stockholm; Visiting Professor at the Universities of Orléans (Fr) and of Reims (Fr). Her research in Biophysics at the University of Milano-Bicocca is focused on the study of protein folding and aggregation in vitro and in situ.MARINA LOTTI, PhD, is Professor of Biochemistry at the University of Milano-Bicocca, Italy, where she leads the group Protein Engineering and Industrial Enzymology, and is the Head of the Department of Biotechnology and Biosciences. She obtained her PhD degree at the Max-Planck Institute of Molecular Genetics, Berlin, and was a researcher of the Italian National Research Council. Major research topics include the production of recombinant proteins, protein aggregation, cold-active enzymes, and intrinsically disordered proteins.

• Contributors viiPreface xiIntroduction to the Wiley Series in Protein and Peptide Science xiii1 Fundamentals of Protein Folding 1Vladimir N. Uversky2 Recruiting Unfolding Chaperones to Solubilize Misfolded Recombinant Proteins 63Rayees U.H. Mattoo and Pierre Goloubinoff3 Osmolytes as Chemical Chaperones to Use in Protein Biotechnology 77Ario de Marco4 Inclusion Bodies in the Study of Amyloid Aggregation 93Anna Villar-Piqué and Salvador Ventura5 Protein Aggregation in Unicellular Eukaryotes 117Marina Caldara, Joris Winderickx, and Vanessa Franssens6 Structural Properties of Bacterial Inclusion Bodies 151Antonino Natalello, Diletta Ami, and Silvia Maria Doglia7 Residue-Specific Structural Studies of Inclusion Bodies 181Christian Wasmer, Marielle Wälti, Yongli Chen, and Lei Wang8 Biomedical Applications of Bacterial Inclusion Bodies 203Imma Ratera, Spela Peternel, Joaquin Seras-Franzoso, Olivia Cano-Garrido, Elena García-Fruitós, Rafael Cubarsí, Esther Vazquez, José Luis Corchero, Escarlata Rodríguez-Carmona, Jaume Veciana, and Antonio Villaverde9 Aggregation of Recombinant Proteins: Understanding Basic Issues to Overcome Production Bottlenecks 221Marina Lotti and Loredano Pollegioni10 Fusion to a Pull-Down Module: Designing Enzymes to Form Biocatalytically Active Insoluble Aggregates 247Bernd NidetzkyIndex 263

SILVIA MARIA DOGLIA, PhD, is Professor of Physics at the University of Milano-Bicocca, Italy. She received her Laurea degree in Physics at the University of Milano. She has been Staff Research Scientist of the Italian National Research Council; Visiting Scientist at the University of Stockholm; Visiting Professor at the Universities of Orléans (Fr) and of Reims (Fr). Her research in Biophysics at the University of Milano-Bicocca is focused on the study of protein folding and aggregation in vitro and in situ.MARINA LOTTI, PhD, is Professor of Biochemistry at the University of Milano-Bicocca, Italy, where she leads the group Protein Engineering and Industrial Enzymology, and is the Head of the Department of Biotechnology and Biosciences. She obtained her PhD degree at the Max-Planck Institute of Molecular Genetics, Berlin, and was a researcher of the Italian National Research Council. Major research topics include the production of recombinant proteins, protein aggregation, cold-active enzymes, and intrinsically disordered proteins.

• Contributors viiPreface xiIntroduction to the Wiley Series in Protein and Peptide Science xiii1 Fundamentals of Protein Folding 1Vladimir N. Uversky2 Recruiting Unfolding Chaperones to Solubilize Misfolded Recombinant Proteins 63Rayees U.H. Mattoo and Pierre Goloubinoff3 Osmolytes as Chemical Chaperones to Use in Protein Biotechnology 77Ario de Marco4 Inclusion Bodies in the Study of Amyloid Aggregation 93Anna Villar-Piqué and Salvador Ventura5 Protein Aggregation in Unicellular Eukaryotes 117Marina Caldara, Joris Winderickx, and Vanessa Franssens6 Structural Properties of Bacterial Inclusion Bodies 151Antonino Natalello, Diletta Ami, and Silvia Maria Doglia7 Residue-Specific Structural Studies of Inclusion Bodies 181Christian Wasmer, Marielle Wälti, Yongli Chen, and Lei Wang8 Biomedical Applications of Bacterial Inclusion Bodies 203Imma Ratera, Spela Peternel, Joaquin Seras-Franzoso, Olivia Cano-Garrido, Elena García-Fruitós, Rafael Cubarsí, Esther Vazquez, José Luis Corchero, Escarlata Rodríguez-Carmona, Jaume Veciana, and Antonio Villaverde9 Aggregation of Recombinant Proteins: Understanding Basic Issues to Overcome Production Bottlenecks 221Marina Lotti and Loredano Pollegioni10 Fusion to a Pull-Down Module: Designing Enzymes to Form Biocatalytically Active Insoluble Aggregates 247Bernd NidetzkyIndex 263

SILVIA MARIA DOGLIA, PhD, is Professor of Physics at the University of Milano-Bicocca, Italy. She received her Laurea degree in Physics at the University of Milano. She has been Staff Research Scientist of the Italian National Research Council; Visiting Scientist at the University of Stockholm; Visiting Professor at the Universities of Orléans (Fr) and of Reims (Fr). Her research in Biophysics at the University of Milano-Bicocca is focused on the study of protein folding and aggregation in vitro and in situ.MARINA LOTTI, PhD, is Professor of Biochemistry at the University of Milano-Bicocca, Italy, where she leads the group Protein Engineering and Industrial Enzymology, and is the Head of the Department of Biotechnology and Biosciences. She obtained her PhD degree at the Max-Planck Institute of Molecular Genetics, Berlin, and was a researcher of the Italian National Research Council. Major research topics include the production of recombinant proteins, protein aggregation, cold-active enzymes, and intrinsically disordered proteins.

• Contributors viiPreface xiIntroduction to the Wiley Series in Protein and Peptide Science xiii1 Fundamentals of Protein Folding 1Vladimir N. Uversky2 Recruiting Unfolding Chaperones to Solubilize Misfolded Recombinant Proteins 63Rayees U.H. Mattoo and Pierre Goloubinoff3 Osmolytes as Chemical Chaperones to Use in Protein Biotechnology 77Ario de Marco4 Inclusion Bodies in the Study of Amyloid Aggregation 93Anna Villar-Piqué and Salvador Ventura5 Protein Aggregation in Unicellular Eukaryotes 117Marina Caldara, Joris Winderickx, and Vanessa Franssens6 Structural Properties of Bacterial Inclusion Bodies 151Antonino Natalello, Diletta Ami, and Silvia Maria Doglia7 Residue-Specific Structural Studies of Inclusion Bodies 181Christian Wasmer, Marielle Wälti, Yongli Chen, and Lei Wang8 Biomedical Applications of Bacterial Inclusion Bodies 203Imma Ratera, Spela Peternel, Joaquin Seras-Franzoso, Olivia Cano-Garrido, Elena García-Fruitós, Rafael Cubarsí, Esther Vazquez, José Luis Corchero, Escarlata Rodríguez-Carmona, Jaume Veciana, and Antonio Villaverde9 Aggregation of Recombinant Proteins: Understanding Basic Issues to Overcome Production Bottlenecks 221Marina Lotti and Loredano Pollegioni10 Fusion to a Pull-Down Module: Designing Enzymes to Form Biocatalytically Active Insoluble Aggregates 247Bernd NidetzkyIndex 263