Polycomb Group Proteins

Methods and Protocols

Häftad, Engelska, 2023

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This volume explores the latest technologies used in the Polycomb Group of proteins field and helps scientists--working on PcG proteins--investigate all functions of PcG proteins in diverse cellular contexts.

Produktinformation

Utgivningsdatum2023-05-23
Mått178 x 254 x 15 mm
Vikt496 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor246
Upplaga2
FörlagSpringer-Verlag New York Inc.
ISBN9781071631454

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Biokemi inom Naturvetenskap och teknik

Analyzing the Genome-Wide Distribution of Histone Marks by CUT&Tag in Drosophila Embryos.- Chromatin Preparation and Chromatin Immuno-Precipitation from Drosophila Heads.- Detecting Cell Compartment Specific PRC2-RNA Interactions via UV-RIP.- Chromosome Conformation Capture Followed by Genome-Wide Sequencing (Hi-C) in Drosophila Embryos.- Physics-Based Polymer Models to Probe Chromosome Structure in Single Molecules.- Co-Immunoprecipitation (Co-IP) in Mammalian Cells.- Site Directed Mutagenesis Protocol to Determine the Role of Amino Acid Residues in Polycomb Group (PcG) Protein Function.- Isolation of Chromatin Proteins by Genome Capture.- Dynamic Interactome of PRC2-EZH1 Complex Using Tandem-Affinity Purification and Quantitative Mass Spectrometry.- Replication Timing of Gene Loci in Different Cell Cycle Phases.- Polycomb Bodies Detection in Murine Fibromuscular Stroma from Skin, Skeletal Muscles, and Aortic Tissues.- Segmentation, 3D Reconstruction, and Analysis of PcG Proteins in Fluorescence Microscopy Images in Different Cell Culture Conditions.- STORM Microscopy and Cluster Analysis for PcG Studies.- Quantitative Analysis of PcG-Associated Condensates by Stochastic Optical Reconstruction Microscopy (STORM).- Immunoelectron Microscopy Methods.- Differentiation of hPSCs to Study PRC2 Role in Cell-Fate Specification and Neurodevelopment.- Analysis of Polycomb Epigenetic Marks in HeLa Spheroids.- Establishment and Maintenance of Human CRC-Derived Organoids for PcGs Studies.