Del i serien Methods in Molecular Biology

Pollen and Pollen Tube Biology

Methods and Protocols

Inbunden, Engelska, 2020

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This volume explores a collection of experimental techniques used to investigate different aspects of pollen development and function, including its role in reaching the ovule and delivering the two sperm cells.

Produktinformation

Utgivningsdatum2020-06-12
Mått178 x 254 x 25 mm
Vikt852 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor332
FörlagSpringer-Verlag New York Inc.
ISBN9781071606711

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Botanik inom Naturvetenskap och teknik
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Pollen Grain Counting Using a Cell Counter.- A Toolkit for teasing Apart the Early Stages of Pollen-Stigma Interactions in Arabidopsis thaliana.- Decapitation Crosses to Test Pollen Fertility Mutations for Defects in Stigma-Style Penetration.- Isolation of the Pistil-Stimulated Pollen Tube Secretome.- Restricted Pollination for Tracing Individual Pollen Tubes in a Pistil.- Semi-In Vivo Assay for Pollen Tube Attraction.- Isolation and Cloning of Suppressor Mutants with Improved Pollen Fertility.- Workflow to Characterize Mutants with Reproductive Defects.- Internally-Controlled Methods to Quantify Pollen Tube Growth and Penetration Defects in Arabidopsis thaliana.- Arabidopsis thaliana Pollen Tube Culture for Multi-Omics Studies.- Large Scale Analysis of Pollen Viability and Oxidative Level using H2DCFDA-Staining Coupled with Flow Cytometry.- Obtaining Mutant Pollen for Phenotypic Analysis and Pollen Tube Dual-Staining.- Galvanotropic Chamber for Controlled Reorientation of Pollen Tube Growth and Simultaneous Confocal Imaging of Intracellular Dynamics.- Analyzing Intracellular Gradients in Pollen Tubes.- Silicone Chambers for Pollen Tube Imaging in Microstructures In Vitro Environments.- Analyzing the Impact of Protein Overexpression on Ca2+ Dynamics and Development in Tobacco Pollen Tubes.- Imaging and Analysis of the Content of Callose, Pectin, and Cellulose in the Cell Wall of Arabidopsis Pollen Tubes Grown In Vitro.- Characterization of Growth Behavior and the Resulting Forces Applied by Pollen Tubes in a 3D Matrix.- Flow Chamber Assay to Image the Response of FRET-Based Nanosensors in Pollen Tubes to Changes in Medium Composition.- Quantification of Mechanical Forces and Physiological Processes Involved in Pollen Tube Growth Using Microfluidics and Microrobotics.- Measuring Exocytosis Rate in Arabidopsis Pollen Tube Using Corrected Fluorescence Recovery afterPhotoconversion (cFRAPc) Technique.- Testing Pollen Tube Proteins for In Vivo Binding to Phosphatidic Acid by n0Butanol Treatment and Confocal Microscopy.