Del 0 - Methods in Molecular Biology

Poliovirus

Methods and Protocols

Inbunden, Engelska, 2016

1 429 kr

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This volume describes the most common laboratory procedures for isolation, identification and characterization of polioviruses used in clinical and research laboratories.

Produktinformation

Utgivningsdatum2016-03-17
Mått178 x 254 x 28 mm
Vikt848 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor341
FörlagHumana Press Inc.
ISBN9781493932917

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An Introduction to Poliovirus: Pathogenesis, Vaccination and the Endgame for Global Eradication.- Poliovirus Laboratory Based Surveillance: An Overview.- Isolation and Characterisation of Enteroviruses from Clinical Samples.- Isolation and Characterisation of Poliovirus in Cell Culture Systems.- Molecular Characterization of Polio from Environmental Samples – ISSP, The Israeli Sewage Surveillance Protocol.- Quality Assurance in the Polio Laboratory. Cell Sensitivity and Cell Authentication Assays.- A Transgenic Mouse Model of Poliomyelitis.- Standardized Methods for Detection of Poliovirus Antibodies.- Molecular Properties of Poliovirus Isolates: Nucleotide Sequence Analysis,Typing by PCR and Real-time RT-PCR.- Isolation and Characterization of Vaccine-Derived Polioviruses, Relevance for the Global Polio Eradication Initiative.- Phylogenetic Analysis of Poliovirus Sequences.- Generation of Infectious Poliovirus with Altered Genetic Information from Cloned cDNA.- A Rapid Method for Engineering Recombinant Polioviruses or Other Enteroviruses.- Methods to Monitor Molecular Consistency of Oral Polio Vaccine.- Methods for the Quality Control of Inactivated Poliovirus Vaccines.- Measuring Poliovirus Antigenicity by Surface Plasmon Resonance. Application for Potency Indicating Assays.- Identification and Analysis of Antiviral Compounds against Poliovirus.