Pluripotent Stem Cells

• Sources and Derivation of Human Embryonic Stem Cells.- A New Chemical Approach to the Efficient Generation of Mouse Embryonic Stem Cells.- A Review of the Methods for Human iPSC Derivation.- Generation of Human Induced Pluripotent Stem Cells by Lentiviral Transduction.- Generation of Induced Pluripotent Stem Cells with CytoTune, a Non-Integrating Sendai Virus.- Generation of Human Induced Pluripotent Stem Cells (hiPSCs) using Episomal Vectors on Defined Essential 8TM Medium Conditions.- Feeder-free Substrates for Pluripotent Stem Cell Culture.- Methods for Culturing Human Embryonic Stem Cells on Feeders.- Methods for Culturing Human Embryonic Stem Cells in a Xeno-Free System.- Directed Differentiation of Human Pluripotent Stem Cells Along the Pancreatic Endocrine Lineage.- Directed Differentiation of Pluripotent Stem Cells to Functional Hepatocytes.- Highly Efficient Directed Differentiation of Human Induced Pluripotent Stem Cells into Cardiomyocytes.- Generation of iPSC Lines from Human Normal and Neoplastic Blood Cells Using Episomal Vectors.- Cellular Characterization of Human Pluripotent Stem Cells.- TaqMan® OpenArray® High Throughput Transcriptional Analysis of Human Embryonic and Induced Pluripotent Stem Cells.- Epigenome Analysis of Pluripotent Stem Cells.- Single Cell Gene Expression Analysis of Pluripotent Stem Cells.- Profiling Stem Cells Using Quantitative PCR Protein Assays.- Labeled Stem Cells as Disease Models and in DrugDiscovery.- Cloning Technologies.- Stable Transfection Using Episomal Vectors to Create Modified Human Embryonic Stem Cells.- Site-Specific Integration in Human ESC Using Jump-In™ TI™ Technology.

From the reviews:“This book is a collection of methods and protocols for the successful generation and characterization of pluripotent stem cells. … It will be helpful to researchers, lab technicians, and students interested in creating pluripotent cells and their commitment to specific cell lineages. … The combination of approachable methods and extremely detailed protocols make this a comprehensive guide for anyone interested in this specific field of research. … the figures, tables, and diagrams clarify the text and make the protocols easy to understand.” (Michele Fornaro, Doody’s Book Reviews, November, 2013)“It is particularly wellcome a book devoted to the basic of the pluripotent stem cells … . All of the chapters are of great value and help in the every day lab working of the stem cell byologist, no doubt … .” (CarloAlberto Redi, European Journal of Histochemistry, Vol. 57, 2013)