Platelet Proteomics

DR. ÁNGEL GARCÍA is currently a Ramón y Cajal Research Fellow at the Pharmacology Department, Universidade de Santiago de Compostela, Spain. His main research focus is in the area of platelet proteomics, with special emphasis in cardiovascular research and platelet signaling studies. He has written over twenty publications in peer-reviewed journals and has been an invited lecturer at the Platelets 2010 International Symposium (Jerusalem, Israel), and the XXII Congress of the International Society on Thrombosis and Haemostasis, held in Boston, United States, in 2009, where he chaired a session on novel approaches to understanding platelet biology. Dr. García was a postdoctoral research associate of the Proteomics Group at the Oxford Glycobiology Institute, University of Oxford (UK), from 2001 to 2005. DR. YOTIS SENIS is currently a British Heart Foundation Research Fellow at the University of Birmingham, United Kingdom. He is a senior member of the Birmingham Platelet Group. Dr. Senis' research interests include the identification of novel platelet surface glycoproteins and determining their biological and molecular functions. He is also interested in the functional roles of protein tyrosine phosphatases in platelets and thrombosis. Dr. Senis has been an invited lecturer at the Platelet 2010 International Symposium (Jerusalem, Israel) and the Gordon Research Conference on the Cell Biology of Megakaryocytes and Platelets (Galveston, Texas). He has written over twenty publications on platelet signaling and thrombosis.

• Foreword xiiiPreface xvAcronyms xviiPart I General Overview: Platelets, Sample Preparation, and Mass Spectrometry-Based Proteomics1 Platelets and Their Role in Thrombotic and Cardiovascular Disease: The Impact of Proteomic Analysis 3Ronald G. Stanley, Katherine L. Tucker, Natasha E. Barrett, and Jonathan M. Gibbins2 Mass-Spectrometry-Based Proteomics: General Overview and Posttranslational Modification Analysis in the Context of Platelet Research 27Julia Maria Burkhart and Albert Sickmann3 Sample Preparation Variables in Platelet Proteomics for Biomarker Research 67Maria Zellner and Rudolf OehlerPart II Analysis of The Platelet Proteome: Global Approaches and Subproteomes4 Two-Dimensional Gel Electrophoresis: Basic Principles and Application to Platelet Signaling Studies 89Ángel García5 The Platelet Membrane Proteome 111Yotis A. Senis6 Proteomics of Platelet Granules, Organelles, and Releasate 139James P. McRedmond7 The Platelet Microparticle Proteome 159David M. Smalley8 N-Terminal Combined Fractional Diagonal Chromatographic (COFRADIC) Analysis of the Human Platelet Proteome 185Francis Impens, Kenny Helsens, Niklaas Colaert, Lennart Martens, Joël Vandekerckhove, and Kris GevaertPart III Integrated “Omics” and Application to Disease9 Serial Analysis of Gene Expression (SAGE) For Studying the Platelet and Megakaryocyte Transcriptome 209Michael G. Tomlinson10 The Application of Microarray Analysis and Its Integration with Proteomics for Study of Platelet-Associated Disorders 231Dmitri V. Gnatenko and Wadie F. Bahou11 Platelet Functional Genomics 253Isabelle I. Salles, Marie N. O’Connor, Daphne C. Thijssen-Timmer, Katleen Broos, and Hans Deckmyn12 Systems Biology to Study Platelet-Related Bleeding Disorders 285Jan-Willem N. Akkerman and Bernard De Bono13 Platelet Proteomics in Transfusion Medicine 321Thomas Thiele, Leif Steil, Uwe Völker, and Andreas Greinacher14 Cardiovascular Proteomics 341Fernando Vivanco, Fernando de la Cuesta, Maria G. Barderas, Irene Zubiri, and Gloria Álvarez-LlamasIndex 381

"Experienced researchers looking for an up-to-date and comprehensive review of this rapidly developing field will find this book a valuable resource.”  (American Society for Mass Spectrometry, 13 December 2011)