Plant Virology

Methods and Protocols

Häftad, Engelska, 2022

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Cutting-edge and practical, Plant Virology: Methods and Protocols is a valuable resource for plant pathologists, microbiologists, virologists, graduate students, and teachers who are interested in learning more about the developments in plant virology research.

Produktinformation

Utgivningsdatum2022-12-16
Mått178 x 254 x 19 mm
Vikt634 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor320
FörlagSpringer-Verlag New York Inc.
ISBN9781071618370

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In Vivo Detection of Double-Stranded RNA by dRBFC Assay.- Developing Rice Mutants using CRISPR/Cas9-Based Genome Editing Technology.- Microscale Thermophoresis Assay: A Powerful Method to Quantify Protein-Nucleic Acid and Protein-Protein Interactions.- Transient Expression-Mediated Gene Silencing in Plants and Suppression of Gene Silencing with Viral Suppressors.- Isolation and Transfection of Plant Mesophyll Protoplasts for Virology Research.- Split-Luciferase Complementation for Analysis of Virus-Host Protein Interactions.- Monitoring Virus Intercellular Movement from Primary Infected Cells to Neighboring Cells in Plants.- Simultaneous Determination and Subcellular Localization of Protein-Protein Interactions in Plant Cells using Bimolecular Fluorescence Complementation Assay.- A Powerful Method for Studying Protein-Protein Interactions in Plants: Co-immunoprecipitation (Co-IP) Assay.- Membrane and Nuclear Yeast Two-Hybrid Systems.- Tag-Based Pull-Down Assay.- Purification of Plasmodesmata-Enriched Fraction for Proteomic Analyses.- Purification and Proteomics Analysis of Phloem Tissues from Virus-Infected Plants.- Rapid and Specific Purification of Argonaute-Small RNA Complexes from Rice for Slicer Activity.- Silencing Specific Genes in Plants Using Virus-Induced Gene Silencing (VIGS) Vectors.- A Versatile Expression Platform in Insects and Cereals Based on a Cytorhabdovirus.- Production of Virus-Free Chrysanthemum morifolium Ramat by Tissue Culture Techniques.- Long-Term Preservation of Plant Viruses in Cryopreserved Shoot Tips.- Establishment of White-Backed Planthopper Cell Lines.- Biolistic Inoculation of Fruit Tress with Full-Length Infectious cDNA Clones of RNA Viruses.- Purification of Total RNAs and Small RNAs from Fruit Tree Leaf Tissues.- Diagnosis of Viral Diseases Using Deep Sequencing and Metagenomics Analyses.- Immunocapture-Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay for Detection of Plant RNA Viruses.- Detection of Tomato spotted wilt virus (TSWV) Infection in Plants using DAS-ELISA and Dot-ELISA.- Tube Capture (TC) RT-PCR and Multiplex RT-PCR for Diagnosis and Characterization of Viruses in Fruit Trees.- Detection of Cucumber green mottle mosaic virus (CGMMV) in Cucurbitaceous Crop Seeds by RT-PCR.- RNA In Situ Hybridization of Detecting Cucumber Mosaic Virus in Shoots of Nicotiana benthamiana Plants.- Transmission Electron Microscopic Methods for Plant Virology.