Plant Reverse Genetics

• Analysis of High-Throughput Sequencing Data.- Identification of Plant MicroRNAs Using Expressed Sequence Tag Analysis.- Microarray Data Analysis.- Setting Up Reverse Transcription Quantitative-PCR Experiments.- Virus-Induced Gene Silencing in Nicotiana benthamiana and Other Plant Species.- Agroinoculation and Agroinfiltration: Simple Tools for Complex Gene Function Analyses.- Full-Length cDNA Overexpressor Gene Hunting System (FOX Hunting System).- Activation Tagging with En/Spm-I/dSpm Transposons in Arabidopsis.- Activation Tagging and Insertional Mutagenesis in Barley.- Methods for Rice Phenomics Studies.- Development of an Efficient Inverse PCR Method for Isolating Gene Tags from T-DNA Insertional Mutants in Rice.- Transposon Insertional Mutagenesis in Rice.- Reverse Genetics in Medicago truncatula Using Tnt1 Insertion Mutants.- Screening Arabidopsis Genotypes for Drought Stress Resistance.- Protein Tagging for Chromatin Immunoprecipitation (ChIP) from Arabidopsis.- Yeast One-Hybrid Screens for Detection of Transcription Factor DNA Interactions.- Plant Metabolomics by GC-MS and Differential Analysis.- Gramene Database: A Hub for Comparative Plant Genomics.

From the reviews:“This book includes a compendium of methods and protocols derived from functional genomics that have been developed during the last decade … . The book is organized in chapters written by leading researchers from different groups … . Large scale phenotyping is also described with protocols suitable for application in different lab conditions. … Gramene database is presented as an example of integrated information … derived from different species, providing details of the information gathered in it as well as available tools for sequence comparisons.” (Ruth Heinz and Norma Paniego, Boletin de Bibleoteca del IMYZA-INTA, Issue 26, June, 2011)