Plant-Pathogen Interactions

• The Use of Protoplasts to Study Innate Immune Responses.- Marker-Exchange Mutagenesis and Complementation Strategies for the Gram-Negative Bacteria Xanthomonas oryzae pv. oryzae.- Whole-Genome Analysis to Identify Type III-Secreted Effectors.- In planta Expression of Oomycete and Fungal Genes.- Use of Nipponbare BAC Clones for Physical Mapping of an R Gene Locus in Rice.- Agrobacterium-Mediated Transformation to Create an Insertion Library in Magnaporthe grisea.- Identification of Components in Disease-Resistance Signaling in Arabidopsis by Map-Based Cloning.- Yeast Two-Hybrid Approaches to Dissecting the Plant Defense Response.- Use of Rolling-Circle Amplification for Large-Scale Yeast Two-Hybrid Analyses.- Preparative Denaturing Isoelectric Focusing for Enhancing Sensitivity of Proteomic Studies.- Use of Massively Parallel Signature Sequencing to Study Genes Expressed During the Plant Defense Response.- Use of Microarray Analysis to Dissect the Plant Defense Response.- Use of Robust-Long Serial Analysis of Gene Expression to Identify Novel Fungal and Plant Genes Involved in Host-Pathogen Interactions.- Analysis of Gene Function in Rice Through Virus-Induced Gene Silencing.- Use of RNA Interference to Dissect Defense-Signaling Pathways in Rice.- Virus-Induced Gene Silencing in Plant Roots.- Methods for Engineering Resistance to Plant Viruses.- Development of Genetically Engineered Resistant Papaya for papaya ringspot virus in a Timely Manner.

From the reviews: "In a highly lucid manner, this book describes new developments in generic techniques and others which are exclusive to plant pathologists. As a result this book will be of interest to seasoned practioners in the field as much as new post-graduate students. ... The presentation of each section is exemplary, with a useful summary, a concise introduction to each section and a step-by-step protocol. ... this is a wide-ranging protocols book that will be useful to plant pathologists at all stages in their careers." (Dr Luis Mur, Molecular Biotechnology, 2007) "The book is divided into 18 chapters ... . this book is an excellent reference for people interested in validating or improving existing lab protocols. ... serve as a basic reference work for those entering into a new research area. ... Texts are clearly written and the tabular format makes it easier to adopt novel methods step-by-step. This makes the book also a valuable tool for people involved in teaching. All in all, I can recommend this book as a good value for the money." (Ulrich Schaffrath, Journal of Plant Physiology, Vol. 165, 2008) "In this volume, the editor has assembled a versatile collection of papers that focus on large-scale analyses of plant-pathogen interactions. ... With contributions from over 40 experts in their fields, this stepwise set of protocols ... will certainly be of interest to molecular plant-pathogen researchers." (Tzvi Tzfira, Quarterly Review of Biology, Vol. 82, 2007)