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With numerous methods available for the quantification ofnitric oxide (NO), this detailed book explores their advantages anddisadvantages in order to provide a foundation for further research in plantnitric oxide.
An Overview of Methods in Plant Nitric Oxide (NO) Research:Why Do We Always Need to Use Multiple Methods?.- Chemiluminescence Detection ofNitric Oxide from Roots, Leaves, and Root Mitochondria.- Nitric Oxide Measurementfrom Purified Enzymes and Estimation of Scavenging Activity by Gas PhaseChemlimunimescence Method.- Localization of Nitric Oxide in Wheat Roots by DAFFluorescence.- Nitric Oxide (NO) Measurements in Stomatal Guard Cells.- Methodsto Detect Nitric Oxide in Plants: Are DAFs Really Measuring NO?.- A NovelProtocol for Detection of Nitric Oxide in Plants.- Detection of Nitric Oxide byElectron Paramagnetic Resonance Spectroscopy: Spin-Trapping withIron-Dithiocarbamates.- Measurement of Nitric Oxide (NO) Generation Rate byChloroplasts Employing Electron Spin Resonance (ESR).- Laser-Based Methods forDetection of Nitric Oxide in Plants.- Electrochemical Detection of Nitric Oxidein Plant Cell Suspensions.- Quantification and Localization of S-Nitrosothiols(SNOs) in Higher Plants.- Analysis of the Expression and Activity of NitricOxide Synthase from Marine Photosynthetic Microorganisms.- Identification ofS-Nitrosothiols by the Sequential Cysteine Blocking Technique.- Detection ofS-Nitrosoglutathione Reductase Activity in Plants.- Detection of Peroxynitritein Plants Exposed to Bacterial Infection.