Del 0 - Methods in Molecular Biology

Plant Mitochondria

Methods and Protocols

Inbunden, Engelska, 2021

3 179 kr

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This detailed volume presents a wide range of techniques for plant mitochondrial analysis, ranging from tried-and-tested work horse techniques to the latest innovations.

Produktinformation

Utgivningsdatum2021-09-21
Mått203 x 259 x 20 mm
Vikt772 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor342
FörlagSpringer-Verlag New York Inc.
ISBN9781071616529

Tillhör följande kategorier

Botanik inom Naturvetenskap och teknik
Växtböcker inom Djur och Natur

Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients.- Cell Type-Specific Isolation of Mitochondria in Arabidopsis.- Isolation of Plant Mitochondria Using Affinity Purification.- Isolation of Highly Purified, Intact, and Functional Mitochondria from Potato Tubers Using a Two-in-One Percoll Density Gradient.- Integrity Assessment of Isolated Plant Mitochondria.- High-Throughput Oxygen Consumption Measurements in Leaf Tissue Using Oxygen Sensitive Fluorophores.- Assessment of Respiratory Enzymes in Intact Cells by Permeabilization with Alamethicin.- Assessing the Kinetics of Metabolite Uptake and Utilization by Isolated Mitochondria Using Selective Reaction Monitoring-Mass Spectrometry (SRM-MS).- Complexome Profiling of Plant Mitochondrial Fractions.- High Throughput BN-PAGE for Mitochondrial Respiratory Complexes.- Assessment of Mitochondrial Protein Composition and Purity by Mass Spectroscopy.- Mitochondrial GFP-Tagged Protein Localization Using Transient Transformations in Arabidopsis thaliana.- Assessment of Mitochondrial Protein Topology and Membrane Insertion.- Assessment of Protein Synthesis in Mitochondria Isolated from Rosette Leaves and Liquid Culture Seedlings of Arabidopsis.- Markers for Mitochondrial ROS Status.- Mass Spectrometry-Based Quantitative Cysteine Redox Proteome Profiling of Isolated Mitochondria Using Differential iodoTMT Labeling.- Analysis of the Plant Mitochondrial Transcriptome.- Quantification of Mitochondrial RNA Editing Efficiency Using Sanger Sequencing Data.- Nascent Transcript Sequencing for the Mapping of Promoters in Arabidopsis thaliana Mitochondria.- Assessment of Mitochondrial DNA Copy Number, Stability, and Repair in Arabidopsis.- Measuring the Activity of DNA Repair Enzymes in Isolated Mitochondria.- MitoTALENs: A Method for Targeted Gene Disruption in Plant Mitochondrial Genomes.