Del 2900 i serien Methods in Molecular Biology

Plant MicroRNAs

Methods and Protocols

Inbunden, Engelska, 2025

AvStefan de Folter

3 139 kr

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This second volume is a collection of new and updated protocols to study miRNA functions in plants.

Produktinformation

Utgivningsdatum2025-05-17
Mått178 x 254 x 26 mm
Vikt908 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor366
Upplaga2
FörlagSpringer-Verlag New York Inc.
ISBN9781071643976

Tillhör följande kategorier

Botanik inom Naturvetenskap och teknik
Växtböcker inom Djur och Natur
Biovetenskap inom Naturvetenskap och teknik

The Use of Arabidopsis thaliana to Characterize the Production and Action Stages of the Plant MicroRNA Pathway.- An overview of miRNA and miRNA target analysis tools.- Prediction of miRNA targets in plants.- An overview of miRNA and miRNA target analysis tools.- Scalable Approach to Evaluate Plant microRNA Trimming and Tailing from Small RNA-Seq data.- In vitro transcription of plant miRNA for structural and processing analysis.- Cost-Effective and Sensitive Probe-Based Universal (PBU) qPCR for MicroRNA Quantification in Plants.- Northern blot analysis of plant microRNAs and other small RNAs.- Northern blot analysis of plant microRNAs and other small RNAs.- Determination of expression patterns of plant miRNAs by Transcriptional Fusion using GUS and Fluorescent reporters.- A Visual Reporter System for Analyzing Small RNA-Triggered Local and Systemic Silencing of an Endogenous Plant Gene.- Unravelling tissue specific miRNAs role in plants exploiting the GAL4/UAS system. -The Luciferase reporter on intronic microRNA-directed regulation.- A simple and efficient protocol to transform and regenerate CRISPR-Cas9-mediated genome edited tomato plants.- CRISPR-activation: boosting expression of plant MIRs.- Gene silencing in plants through exogenous application of miRNAs.- A strategy to use Artificial MicroRNAs for Gene silencing and Overcome (AmiGO).- Degradome sequencing.- Whole mount staining and immunolocalization procedures for developing ovules of Vigna unguiculata.- Bioinformatic enrichment and network analyses of miRNA-related high-throughput sequencing data.- Identifying cis-regulatory binding sites in promoter regions of miRNA genes: A step-by-step protocol.- A Detailed Protocol for the Isolation of Arabidopsis thaliana Mesophyll Protoplasts and their Applications.- A protocol for live imaging of Arabidopsis gynoecium development using confocal microscopy.