This second volume provides updated and new methods on plant–biotic interactions, including the use of bioinformatics as a tool, as well as various protocols from molecular biology, biochemistry, physiology, pathology, and agricultural sciences. Written in the format of the Methods and Protocols in Food Science series, chapters list necessary materials and methods for readily reproducible protocols.Authoritative and cutting-edge, Plant Biotic Stress: Methods and Protocols, Volume 2 aims to provide well-established protocols along with recent methodological advancements, enabling accurate, reproducible, and in-depth research, particularly in the context of crop protection.