Del i serien Methods in Molecular Biology

Photosynthesis

Methods and Protocols

Inbunden, Engelska, 2018

2 519 kr

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Broken into four sections, it explores the measurement of physiological photosynthetic parameters, quantifying photosynthetic enzyme abundance and catalytic activity, visualizing cellular and sub-cellular phenotypes, and photosynthesis-inspired energy generation.

Produktinformation

Utgivningsdatum2018-07-06
Mått178 x 254 x 25 mm
Vikt972 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor355
Upplaga18001
FörlagHumana Press Inc.
ISBN9781493977857

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Survey of Tools for Measuring In Vivo Photosynthesis.- Photosynthetic Gas Exchange in Land Plants at the Leaf Level.- Design and Use of a Digitally-Controlled Device for Accurate, Multiplexed Gas Exchange Measurements of the Complete Foliar Parts of Plants.- Measuring Canopy Gas Exchange Using CAnopy Photosynthesis and Transpiration Systems (CAPTS).- Light-Response Curves in Land Plants.- Chlorophyll Fluorescence on the Fast Timescale.- Sub-Saturating Multiphase Flash Irradiances to Estimate Maximum Fluorescence Yield.- Chlorophyll Fluorescence Imaging.- Measurement of O2 Uptake and Evolution in Leaves In Vivo Using Stable Isotopes and Membrane Inlet Mass Spectrometry.- Using Carbon Stable Isotopes to Study C3 and C4 Photosynthesis: Models and Calculations.- Liquid-Phase Measurements of Photosynthetic Oxygen Evolution.- Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting.- Extraction of Rubisco to Determine Catalytic Constants.- Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts.- Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features.- Determining the Subcellular Localization of Fluorescently-Tagged Proteins Using Protoplasts Extracted from Transiently-Transformed Nicotiana benthamiana Leaves.- 3D Clearing and Molecular Labeling in Plant Tissues.- Evaluation of Lipids for the Study of Photosynthetic Membranes.- “Click” Methodology for the Functionalization of Water Oxidation Catalyst Iridium Oxide Nanoparticles with Hydrophobic Dyes for Artificial Photosynthetic Constructs.- Biophotovoltaics: Design and Study of Bio-Electrochemical Systems for Biotechnological Applications and Metabolic Investigation.