Phage Display

Methods and Protocols

Inbunden, Engelska, 2023

Av Michael Hust, Theam Soon Lim

4 519 kr

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This second edition details new and updated methods on different antibody libraries, along with novel approaches for antibody discovery.

Produktinformation

Utgivningsdatum2023-09-08
Mått178 x 254 x 39 mm
Vikt1 354 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor605
Upplaga2
FörlagSpringer-Verlag New York Inc.
ISBN9781071633809

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Tillhör följande kategorier

Antibody phage display.- Construction of human immune and naive scFv Phage Display libraries.- Construction of Naïve and Immune Human Fab Phage Display Library.- Construction of synthetic antibody phage display libraries.- Construction of chicken and ostrich antibody libraries.- Construction of Rabbit Immune Antibody Libraries.- Isolation and characterization of single-domain antibodies from immune phage display libraries.- Phagekines: directed evolution and characterization of functional cytokines displayed on phages.- Efficient Cloning of Inserts for Phage Display by Golden Gate Assembly.- Construction of an ultra-large phage display library by Kunkel mutagenesis and rolling circle amplification.- Construction of semi-synthetic shark vNAR yeast surface display antibody libraries.- Antibody selection via Phage Display in microtiter plates.- Antibody selection in solution using magnetic beads.- Streptavidin Coated Solid Phase Extraction (SPE) TipsFor Antibody Phage Display Biopanning.- Magnetic Nanoparticle-Based Semi-Automated Panning for High-throughput Antibody Selection.- Antibody selection on cells targeting membrane proteinsTargeting Intracellular Antigens With pMHC-binding Antibodies: A Phage Display Approach.- Antibody Isolation from a Human Synthetic Libraries of Single-chain Antibodies and analysis using NGS.- Selection of affibody affinity proteins from phagemid libraries.- Antibody affinity and stability maturation by error-prone PCR.- Antibody batch cloning.- Deep Mining of Complex Antibody Phage Pools.- High-throughput IgG reformatting and expression using hybrid secretion signals and InTag positive selection technology.- Validation and the Determination of Antibody Bioactivity Using MILKSHAKE and Sundae Protocols.- Mapping polyclonal antibody responses to infection using next generation phage display.- Applications of high-throughput DNA sequencing to single-domain antibodydiscovery and engineering.- Biomarker discovery by ORFeome Phage Display.- Mapping Epitopes by Phage Display.- Epivolve: A Protocol for Site-directed Antibodies.