PCR Strategies

Inbunden, Engelska, 1995

Av Michael A. Innis, David H. Gelfand, John J. Sninsky

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PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a completely new set of up-to-date strategies and protocols for getting the most from PCR.

The editors have organized the book into four sections, focusing on principles, analyses, research applications, and alternative strategies for a wide variety of basic and clinical needs. If you own PCR Protocols, you will want PCR Strategies. If you don't own PCR Protocols, you will want to buy both!

Concepts explained
Methods detailed
Trouble-shooting emphasized
Novel applications highlighted
Key concepts for PCR
Analysis of PCR products
Research applications
Alternative amplification strategies

Produktinformation

Utgivningsdatum1995-07-06
Mått152 x 229 x undefined mm
Vikt770 g
FormatInbunden
SpråkEngelska
Antal sidor373
FörlagElsevier Science
ISBN9780123721822

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Principles of PCR Amplification: PrefaceThe Use of Co-Solvents to Enhance Amplification By the Polymerase Chain ReactionDNA Polymerase Fidelity: Misinsertion and Mismatch ExtensionExtraction of Nucleic Acids: Sample Preparation from Paraffin-Embedded TissuesThermostable DNA PolymerasesDirect RNA AmplificationNucleic Acid Hybridization and Unconventional BasesMolecular Phylogentic AnalysisThe Dutp-Uracil Glycosylase System for Caryover Control and Enhanced SpecificityQuantitation. Analysis of PCR Products: Carrier Detection of Cystic Fibrosis Mutations Using PCR-Amplified DNA and a Mismatch-Binding Protein, MutsSingle-Stranded Conformational Polymorphisms (SSCP)Analysis of PCR Products By Covalent Reverse Dot Blot Hybridization. HPLC Analysis of PCR ProductsRare Mutation Detection. Heteroduplex Mobility Shift Assays for Phylogenetic AnalysisConsiderations for Use of RNA: Amplified DNA Hybrid Detection Using a Monoclonal Antibody ConjugatePCR Amplification of VntrsSite-Specific Mutagenesis Using the Polymerase Chain Reaction. Exact Quantification of DNA/RNA Copy Numbers By PCR/TGGE. PCR in Situ: Amplification and Detection of DNA in a Cellular ContextChromosome Specific PCR: Maternal BloodDNA and RNA Fingerprinting Using Arbitrarily Primed PCRPCR-Based Screening of Yeast Artificial Chromosome (YAC) LibrariesOligonucleotide Ligands that Discriminate Between Theophylline and CaffeineGeneration of Single Chain Antibody Fragments By PCR. Longer PCR AmplificationsRapid Screening for Mutations Using Intercalating DyesDirect Analysis of Specific Bands from Arbitrarily Primed PCR ReactionDetection of Leber's Hereditary Optic Neuropathy By Nonradioactive-LCRDetection of Listeria Monocytogenes By PCR-Coupled Ligase Chain Reaction (LCR)3SR as an Amplification Strategy for RNA