PCR Protocols

• Setup of a PCR Laboratory.- Long Range PCR with a DNA Polymerase Fusion.- Isolation of Genomic Insertion Sites of Proviruses Using Splinkerette-PCR-Based Procedures.- Lariat-Dependent Nested PCR for Flanking Sequence Determination.- CODEHOP PCR and CODEHOP PCR Primer Design.- Sequencing of Difficult DNA Regions by SAM Sequencing.- A Global Single-Cell cDNA Amplification Method for Quantitative Microarray Analysis.- Quantitation of MicroRNAs by Real-Time RT-qPCR.- High-Throughput SuperSAGE.- Deep Cap Analysis of Gene Expression (Deep CAGE).- Linking Emulsion PCR Haplotype Analysis.- PAP-LMPCR: An Improved, Sequence-Selective Method for the In vivo Analysis of Transcription Factor Occupancy and Chromatin Fine Structure.- The Many Faces of MLPA.- Assessing Gene-Specific Methylation Using HRM-Based Analysis.- Alu PCR.- Asynchronous PCR.- Novel Applications of PCR through the Use of DNA Substrates.- Enhanced Solid Phase PCR for Increased Loading of Amplicon onto Solid Support.- Application of Blocking Oligonucleotides to Improve Signal to Noise Ratio in a PCR.- Asymmetric Overlap Extension PCR Method for Site-Directed Mutagenesis.- Ribosome Display: A Technology for Selecting and Evolving Proteins from Large Libraries.- GLOBE: Analysis of DNA-Protein Interaction Analysis.- PCR DNA-Array Profiling of DNA-Binding Transcription Factor Activities in Adult Mouse Tissues.- Nucleotide Exchange and Excision Technology (NExT) DNA Shuffling and Directed Evolution.

From the reviews of the third edition: "This book describes recently developed tools such as PCR cloning and sequencing, expression ... and other important techniques crucial for all PCR methods. ... Students and researchers interested in learning latest methods in PCR will find this book extremely useful. ... This book presents cutting-edge methodologies crucial for all PCR methods. Given the rapid advances in the field of PCR methods, this third edition is essential and justifies replacement of the previous edition." (Omer Iqbal, Doody's Book Reviews, February, 2012) "The book consists of 5 parts and 24 chapters. ... Third edition of 'PCR Protocols' represents very useful handbook written in form of original and modified PCR protocols. Since it provides suggestions for solution of basic research as well as clinical and diagnostic problems, it will probably find its place in many molecular biologylaboratories." (Lubica Lacinova, General Physiology and Biochemistry, May, 2011)