Patch-Clamp Methods and Protocols

• History of Electrophysiology and the Patch-Clamp.- Whole-Cell Patch-Clamp Analysis of Recombinant NMDA Receptor Pharmacology Using Brief Glutamate Applications.- Patch-Clamp Combined with Voltage/Concentration Clamp to Determine the Kinetics and Voltage Dependency of N-methyl-D-aspartate (NMDA) Receptor Open Channel Blockers.- State-of-the-art Automated Patch-Clamp: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening.- Industrializing Electrophysiology: HT Automated Patch-Clamp on SyncroPatch® 96 Using Instant Frozen Cells.- Planar Patch Clamp for Neuronal Networks–Considerations and Future Perspectives.- Methods for the Study of Synaptic Receptor Functional Properties.- Single-Cell RT-PCR, A Technique to Decipher the Electrical, Anatomical and Genetic Determinants of Neuronal Diversity.- Whole-Cell Voltage-Clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique.- Multiphoton Imaging Approaches for Studying Striatal Dendritic Excitability.- Investigation of Synaptic Microcircuits Using Paired Patch-Clamp Recordings in Acute Brain Slices.- Outside-Out ‘Sniffer-patch’ Clamp Technique for in situ Measures of Neurotransmitter Release.- A Cost Effective Method for Preparing, Maintaining and Transfecting Neurons in Organotypic Slices.- Acute Brain Slice Methods for Adult and Aging Animals: Application of Targeted Patch Clamp Analysis and Optogenetics.- Synaptic Connectivity in Engineered Neuronal Networks.- Modeling of Action Potential Generation in NG108-15 Cells.- Whole-Cell Patch-Clamp Recordings in Freely Moving Animals.- Pressure-Polished Borosilicate Pipettes are “Universal Sealer” Yielding Low Access Resistance and Efficient Intracellular Perfusion.- Current Recordings at the Single Channel Level in Adult Mammalian Isolated Cardiomyocytes.- Dynamic Clamp as a Tool to Study the Functional Effects of Individual Membrane Currents.- Dynamic Clamp in Cardiac and Neuronal Systems Using RTXI.- Ion Selectivity of Pore-Forming Peptides and Ion Channels Measured in Xenopus Oocytes.- Principles of Single-Channel Kinetic Analysis.

“This is a very good up to date ‘how to’ manual with dozens of unique protocols regarding single channel, whole cell, and network patch recordings. … This atlas and textbook is a good choice for ‘patchers’, neurohysiologists, biophysicists, students, fellows and ‘cell’ molecular physiologists.” (Joseph J. Grenier, Amazon.com, September, 2015)