Oxidants and Antioxidants

• Techniques for Histological Identification.- Cytochemical Localization of H2O2 in Biological Tissues.- Localization of Intracellular Lipid Hydroperoxides Using The Tetramethylbenzidine Reaction for Transmission Electron Microscopy.- The Immunohistochemical Localization of Glutathione Peroxidase.- 4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody.- Immunohistochemical Detection of Protein Oxidation.- Indirect Immunofluorescence Detection of Protein-Bound 4-Hydroxynonenal in Tissue Sections and Isolated Cells.- Techniques for Subcellular Localization.- Methods for Studying the Binding of Advanced Glycated Proteins to Receptors for Advanced Glycation Endproducts (AGE Receptors).- Measurement of Blue Fluorescence as a Protein Marker for Oxidized Membranes.- X-Ray Diffraction Analysis of Membrane Structure Changes with Oxidative Stress.- Detection of Estrogen Receptor by In Situ Hybridization.- Tumor Necrosis Factor-? Quantification and Expression by In Situ Hybridization.- Techniques for Molecular Biology.- Translocation of p47phox and Activation of NADPH Oxidase in Mononuclear Cells.- Activation of Nuclear Factor-?B (NF-?B) in Mononuclear Cells (MNC).- Assay of Intracellular Hydrogen Peroxide Generation in Activated Cytometry Individual Neutrophils by Flow.- DNA-Binding Activity of Hypoxia-Inducible Factors (HIFs).- Analysis of Heat-Shock Transcription Factor and Element-Binding Activity.- Slot-Blot Hybridization in Studying Gene Expression During Oxygen Deprivation.- An Expression System for a Transporter of Iron and Other Metals.- Analysis of Gene Expression Following Oxidative Stress.- Measurement of Immunoglobulin G Oxidation by Western-Blot Analysis.- Thioredoxin and Redox Regulation of the Nuclear Receptor.- Analysis of Phospholipid HydroperoxideGlutathione Peroxidase mRNA.- Expression of Human Phospholipid Hydroperoxide Glutathione Peroxidase.- Techniques for In Vivo/Ex Vivo/In Vitro Applications.- Superoxide Production in the Islet of Langerhans Detected by the MCLA Chemiluminescence Method.- In Vivo Detection of Transition Metals and Nitrosyl-Heme Complexes Using Ex Vivo Electron Paramagnetic Resonance Spectroscopy.- Spectral-Spatial Electron Paramagnetic Resonance Imaging (EPRI) in Skin Biopsies at 9.5 GHz.- In Vivo In Situ Detection of Nitric Oxide Using Low-Frequency EPR Spectroscopy.- of NOS II Gene into Primary Cultures of Bovine and Human Endothelial Cells.- Detection of Peroxynitrite-Induced Protein and DNA Modifications.- Immunochemical Detection of a Fluorophore Derived from the Lipid Peroxidation Product 4-Hydroxy-2-Nonenal and Lysine.- Liver Slice Technology as an In Vitro Model for Metabolic and Toxicity Studies.- Cytofluorescence Techniques for the Visualization of Distinct Pools of Protein Thiols at the Single Cell Level.- Preparation of Microspheres and Incorporation of Lipid Hydroperoxide for Sustained Release Studies.- Measurement of Carotenoids in the Living Primate Eye Using Resonance Raman Spectroscopy.- Interstitial Photodynamic Therapy with Moving Exposure Fiber.- Statistical Analysis.

"...this volume is unique on its focus on protocols for identification and localization of oxidants, using molecular biology and other types of applications both in-vitro and in-vivo. This book is recommended for all biomedical research libraries and researchers at all levels who are interested in oxidative stress measurement." - E-STREAMS