Oral Biology

• Saliva Studies.- Gene Therapy of Salivary Diseases.- Collection, Storage, and Processing of Saliva Samples for Downstream Molecular Applications.- Proteomic Analysis of Saliva: 2D Gel Electrophoresis, LC-MS/MS, and Western Blotting.- Transcriptomic Analyses of Saliva.- Oral Microbiology.- The Oral Microbiota: General Overview, Taxonomy, and Nucleic Acid Techniques.- Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE).- Protocols to Study the Physiology of Oral Biofilms.- Adhesion of Yeast and Bacteria to Oral Surfaces.- Quantitative Analysis of Periodontal Pathogens by ELISA and Real-Time Polymerase Chain Reaction.- Bacterial Viability Determination in a Dentinal Tubule Infection Model by Confocal Laser Scanning Microscopy.- Characterization of Anti-competitor Activities Produced by Oral Bacteria.- Natural Transformation of Oral Streptococci.- Use of In Vivo-Induced Antigen Technology (IVIAT) to Identify Virulence Factors of Porphyromonas gingivalis.- Oral Bacterial Genome Sequencing Using the High-Throughput Roche Genome Sequencer FLX System.- Use of a Yeast-Based Membrane Protein Expression Technology to Overexpress Drug Resistance Efflux Pumps.- Cells and Tissues.- Explant Culture of Embryonic Craniofacial Tissues: Analyzing Effects of Signaling Molecules on Gene Expression.- A Method to Isolate, Purify, and Characterize Human Periodontal Ligament Stem Cells.- Preclinical Methods for the Evaluation of Periodontal Regeneration In Vivo.- Proteomic Analysis of Dental Tissue Microsamples.- Immunological Techniques: ELISA, Flow Cytometry, and Immunohistochemistry.- Analysis of Immune Responses to Purified Recombinant Antigens of Periodontal Pathogens.- Single-Strand ConformationPolymorphism Analysis for the Diagnosis of T-Cell Clonality in Periodontal Disease.- Real-Time PCR Focused-Gene Array Profiling of Gingival and Periodontal Ligament Fibroblasts.- The Use of Gene Arrays in Deciphering the Pathobiology of Periodontal Diseases.- Bioinformatics Techniques in Microarray Research: Applied Microarray Data Analysis Using R and SAS Software.

From the reviews:“This book comprehensively reviews a wide range of techniques and protocols from diverse areas of oral biology, encompassing current research on microbes, host tissues and saliva. The book is obviously aimed at active researchers in the various fields, but because the research topics are so diverse, I would recommend purchase by institutes and departments … .” (Adam Roberts, Microbiology Today, May, 2011)