The objectives of this project were to (1) develop and evaluate a carbohydrate ligand-binding assay for the purification of noroviruses from reverse transcriptase-polymerase chain reaction (RT-PCR) interfering substances; (2) develop a real-time RT-PCR assay for noroviruses; (3) compare and evaluate previously published and recently developed primer pairs targeting different regions of the norovirus genome in a conventional RT-PCR assay; and (4) use the developed methods for concentration, purification, and molecular detection to examine environmental samples (e.g., shellfish, source and finished water, sewage).?

In this study, the research team developed and evaluated novel methods for the concentration, detection, and genotyping of noroviruses from complex environmental matrices including (1) a novel assay based on H-type 1 histo-bloodgroup carbohydrate bound to magnetic beads, (2) a broadly reactive one-step TaqMan? RT-PCR assay for the detection of GI and GII noroviruses, and (3) hemi-nested conventional RT-PCR assay for genotyping of low-copy number of noroviruses. These methods were applied on naturally contaminated shellfish, raw and finished water, and sewage. The results indicated that the TaqMan? real-time RT-PCR assay is a superior method for rapid and simple monitoring of environmental waters for noroviruses.

The objectives of this project were to (1) develop and evaluate a carbohydrate ligand-binding assay for the purification of noroviruses from reverse transcriptase-polymerase chain reaction (RT-PCR) interfering substances; (2) develop a real-time RT-PCR assay for noroviruses; (3) compare and evaluate previously published and recently developed primer pairs targeting different regions of the norovirus genome in a conventional RT-PCR assay; and (4) use the developed methods for concentration, purification, and molecular detection to examine environmental samples (e.g., shellfish, source and finished water, sewage).?

In this study, the research team developed and evaluated novel methods for the concentration, detection, and genotyping of noroviruses from complex environmental matrices including (1) a novel assay based on H-type 1 histo-bloodgroup carbohydrate bound to magnetic beads, (2) a broadly reactive one-step TaqMan? RT-PCR assay for the detection of GI and GII noroviruses, and (3) hemi-nested conventional RT-PCR assay for genotyping of low-copy number of noroviruses. These methods were applied on naturally contaminated shellfish, raw and finished water, and sewage. The results indicated that the TaqMan? real-time RT-PCR assay is a superior method for rapid and simple monitoring of environmental waters for noroviruses.

The objectives of this project were to (1) develop and evaluate a carbohydrate ligand-binding assay for the purification of noroviruses from reverse transcriptase-polymerase chain reaction (RT-PCR) interfering substances; (2) develop a real-time RT-PCR assay for noroviruses; (3) compare and evaluate previously published and recently developed primer pairs targeting different regions of the norovirus genome in a conventional RT-PCR assay; and (4) use the developed methods for concentration, purification, and molecular detection to examine environmental samples (e.g., shellfish, source and finished water, sewage).?

In this study, the research team developed and evaluated novel methods for the concentration, detection, and genotyping of noroviruses from complex environmental matrices including (1) a novel assay based on H-type 1 histo-bloodgroup carbohydrate bound to magnetic beads, (2) a broadly reactive one-step TaqMan? RT-PCR assay for the detection of GI and GII noroviruses, and (3) hemi-nested conventional RT-PCR assay for genotyping of low-copy number of noroviruses. These methods were applied on naturally contaminated shellfish, raw and finished water, and sewage. The results indicated that the TaqMan? real-time RT-PCR assay is a superior method for rapid and simple monitoring of environmental waters for noroviruses.