The bookpresents an integrated and forward-looking exploration of emerging biological risks at the human–animal–environment interface. Structured across three thematic parts, the book brings together contemporary research, policy perspectives, and applied frameworks essential for understanding and managing complex health challenges through the One Health approach.Part I focuses on microbial and viral frontiers, addressing preparedness for future zoonotic viruses, spillover dynamics influenced by climate change, and model diseases such as rabies and lumpy skin disease. Cutting-edge topics including omics-driven diagnostics, multi-epitope vaccine design, and the role of small ruminants in nutrition under infectious disease pressure are critically examined, highlighting innovation and resilience in One Health systems.Part II is devoted to antimicrobial resistance (AMR), covering molecular mechanisms such as integrons, AMR in enteric bacteria, and comprehensive stewardship strategies. The section emphasizes systems thinking and end-to-end stewardship across human, veterinary, and environmental sectors.Part III addresses food safety, policy, and implementation pathways, with chapters on bacteriophages, natural antimicrobials, food systems as drivers of viral spillover, leptospirosis control, and India-specific One Health policies and pandemic preparedness.Written for postgraduate students, researchers, policymakers, veterinarians, public health professionals, and food safety experts, the book aligns with global and Indian One Health priorities, offering actionable insights for preparedness, prevention, and sustainable health governance.