Del 0 - Methods in Molecular Biology

Nucleic Acid Aptamers

Selection, Characterization, and Application

Inbunden, Engelska, 2022

AvGünter Mayer,Marcus M. Menger,Gunter Mayer

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This updated book reflects improvements in a variety of techniques used to study the aptamer field.

Produktinformation

Utgivningsdatum2022-09-27
Mått178 x 254 x 22 mm
Vikt758 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor282
Upplaga2
FörlagSpringer-Verlag New York Inc.
ISBN9781071626948

Tillhör följande kategorier

Automated ssDNA SELEX Using Histidine Tagged Target Proteins.- Discovery of Aptamers against Cell Surface Markers Using Ligand-Guided Selection.- Implementation of Emulsion PCR for Amplification of Click-Modified DNA during SELEX.- Preparation of Chemically Modified DNA Library for SELEX via Incorporation of CLB-dUTP in Primer Extension Reaction.- RNA Capture-SELEX on Streptavidin Magnetic Beads.- The Bioinformatics of Aptamers: HT-SELEX Analysis with AptaSUITE.- Improvement of Aptamers by High-Throughput Sequencing of Doped-SELEX.- Analysis of Aptamer-Small Molecule Binding Interactions Using Isothermal Titration Calorimetry.- Determination of Aptamer Structure Using Circular Dichroism Spectroscopy.- Immobilization-Free Determination of Dissociation Constants Independent of Ligand Size Using MicroScale Thermophoresis.- Label-Free Determination of the Kinetic Parameters of Protein-Aptamer Interaction by Surface Plasmon Resonance.- Probing of Fluorogenic RNA Aptamers via SupramolecularFörster Resonance Energy Transfer with Universal Fluorescent Nucleobase Analog.- Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT.- Aptamer-Field-Effect Transistors for Small-Molecule Sensing in Complex Environments.- Aptasensor for Impedimetric Detection of Lysozyme.- Biophysical Analysis of Small Molecule Binding to Viral RNA Structures.- Designing and Expressing Circular RNA Aptamers to Regulate Mammalian Cell Biology.- Enzyme-Linked OligoNucleotide Assay (ELONA).- High-Throughput Development and Optimization of RNA-Based Fluorogenic Biosensors of Small Molecules Using Droplet-Based Microfluidics.- Robotic APTamer-Enabled Electrochemical Reader (RAPTER) System for Automated Aptamer-Mediated Electrochemical Analysis.